Quantification of plasma phospholipids by ultra performance liquid chromatography tandem mass spectrometry

We describe a fast and robust ultra performance liquid chromatography tandem mass spectrometry method for the quantification of phospholipid (PL) species in EDTA-plasma samples. We quantified total phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), and sphingomye...

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Veröffentlicht in:Analytical and bioanalytical chemistry 2011-08, Vol.401 (3), p.891-899
Hauptverfasser: Rabagny, Yannick, Herrmann, Wolfgang, Geisel, Jürgen, Kirsch, Susanne H., Obeid, Rima
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Sprache:eng
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Zusammenfassung:We describe a fast and robust ultra performance liquid chromatography tandem mass spectrometry method for the quantification of phospholipid (PL) species in EDTA-plasma samples. We quantified total phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), and sphingomyelin (SM) and several species within these classes using one or two external calibrators and one internal standard for each class. Inter-assay coefficients of variation were
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-011-5154-5