Use of various nuclei as probes in a new NMR method for obtaining proton/deuteron fractionation data

An extension to the new NMR spectroscopic method for rapidly and conveniently obtaining isotopic fractionation factors of substrates undergoing rapid proton-deuteron exchange, with solvent water (H/sub 2/O, HOD, D/sub 2/O), through the observation of /sup 13/C spectra is presented. The intrinsic isotope shift of nuclei near the sites of isotopic incorporation is the basis of this procedure, which has the unique capability of simultaneously measuring the exchange constants for isotopic exchange of several different groups in the same molecule. In this report, /sup 1/H, /sup 7/Li, /sup 11/B, /sup 14/N, /sup 17/O, /sup 19/F, /sup 23/Na, /sup 31/P, /sup 35/Cl, /sup 37/Cl, /sup 39/K, /sup 79/Br, /sup 81/Br, and /sup 127/I are examined and the utility of each as probes in the application of this technique is evaluated. A wider variety of compounds have thus been opened to experimental investigations, using this new method.