Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells

Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of natural products (Washington, D.C.) D.C.), 2012-01, Vol.75 (1), p.93-97
Hauptverfasser: Figueroa-González, Gabriela, Jacobo-Herrera, Nadia, Zentella-Dehesa, Alejandro, Pereda-Miranda, Rogelio
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 97
container_issue 1
container_start_page 93
container_title Journal of natural products (Washington, D.C.)
container_volume 75
creator Figueroa-González, Gabriela
Jacobo-Herrera, Nadia
Zentella-Dehesa, Alejandro
Pereda-Miranda, Rogelio
description Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy.
doi_str_mv 10.1021/np200864m
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_918579235</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>918579235</sourcerecordid><originalsourceid>FETCH-LOGICAL-a413t-2a40220a6873ca61f166cc5175ae464aee169c12afb65d13131ac25bc00997633</originalsourceid><addsrcrecordid>eNp90E1LwzAcBvAgipvTg19AchH1UM1726MW3YQNYei5pGk6OtpkJq3Qb2_m5k4iOSTh_8tDeAC4xOgeI4IfzIYglAjWHoEx5gRFAhF-DMYICxrRMBiBM-_XCCGKUn4KRoRglrCYj0G-1F_aedlAW8FF33R16foVXGpf-04apWExwIV1pjYrOG2sG35mJpwHZX1dag_Dbda30sAnp6XvYLZ952Cmm8afg5NKNl5f7PcJ-Hh5fs9m0fxt-po9ziPJMO0iIhkiBEmRxFRJgSsshFIcx1xqJpjUGotUYSKrQvAS07CkIrxQCKVpLCidgJtd7sbZz177Lm9rr8IPpNG293mKEx6nhPIgb_-VoVGKGGZcBHq3o8pZ752u8o2rW-mGgLYO54fmg73ax_ZFq8uD_K06gOsdkMrna9s7E_r4I-gbsemItA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1023041456</pqid></control><display><type>article</type><title>Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Figueroa-González, Gabriela ; Jacobo-Herrera, Nadia ; Zentella-Dehesa, Alejandro ; Pereda-Miranda, Rogelio</creator><creatorcontrib>Figueroa-González, Gabriela ; Jacobo-Herrera, Nadia ; Zentella-Dehesa, Alejandro ; Pereda-Miranda, Rogelio</creatorcontrib><description>Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy.</description><identifier>ISSN: 0163-3864</identifier><identifier>EISSN: 1520-6025</identifier><identifier>DOI: 10.1021/np200864m</identifier><identifier>PMID: 22148475</identifier><language>eng</language><publisher>United States: American Chemical Society and American Society of Pharmacognosy</publisher><subject>Antineoplastic Agents, Phytogenic - chemistry ; Antineoplastic Agents, Phytogenic - isolation &amp; purification ; Antineoplastic Agents, Phytogenic - pharmacology ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; Drug Resistance, Multiple - drug effects ; Drug Resistance, Neoplasm - drug effects ; Drug Screening Assays, Antitumor ; Female ; Glycosides - chemistry ; Glycosides - isolation &amp; purification ; Glycosides - pharmacology ; Humans ; Ipomoea nil - chemistry ; Molecular Structure ; Rhodamines ; Vinblastine - pharmacology</subject><ispartof>Journal of natural products (Washington, D.C.), 2012-01, Vol.75 (1), p.93-97</ispartof><rights>Copyright © 2011 American Chemical Society and American Society of Pharmacognosy</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a413t-2a40220a6873ca61f166cc5175ae464aee169c12afb65d13131ac25bc00997633</citedby><cites>FETCH-LOGICAL-a413t-2a40220a6873ca61f166cc5175ae464aee169c12afb65d13131ac25bc00997633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/np200864m$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/np200864m$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22148475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Figueroa-González, Gabriela</creatorcontrib><creatorcontrib>Jacobo-Herrera, Nadia</creatorcontrib><creatorcontrib>Zentella-Dehesa, Alejandro</creatorcontrib><creatorcontrib>Pereda-Miranda, Rogelio</creatorcontrib><title>Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells</title><title>Journal of natural products (Washington, D.C.)</title><addtitle>J. Nat. Prod</addtitle><description>Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy.</description><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>Antineoplastic Agents, Phytogenic - isolation &amp; purification</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>Drug Resistance, Multiple - drug effects</subject><subject>Drug Resistance, Neoplasm - drug effects</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Female</subject><subject>Glycosides - chemistry</subject><subject>Glycosides - isolation &amp; purification</subject><subject>Glycosides - pharmacology</subject><subject>Humans</subject><subject>Ipomoea nil - chemistry</subject><subject>Molecular Structure</subject><subject>Rhodamines</subject><subject>Vinblastine - pharmacology</subject><issn>0163-3864</issn><issn>1520-6025</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90E1LwzAcBvAgipvTg19AchH1UM1726MW3YQNYei5pGk6OtpkJq3Qb2_m5k4iOSTh_8tDeAC4xOgeI4IfzIYglAjWHoEx5gRFAhF-DMYICxrRMBiBM-_XCCGKUn4KRoRglrCYj0G-1F_aedlAW8FF33R16foVXGpf-04apWExwIV1pjYrOG2sG35mJpwHZX1dag_Dbda30sAnp6XvYLZ952Cmm8afg5NKNl5f7PcJ-Hh5fs9m0fxt-po9ziPJMO0iIhkiBEmRxFRJgSsshFIcx1xqJpjUGotUYSKrQvAS07CkIrxQCKVpLCidgJtd7sbZz177Lm9rr8IPpNG293mKEx6nhPIgb_-VoVGKGGZcBHq3o8pZ752u8o2rW-mGgLYO54fmg73ax_ZFq8uD_K06gOsdkMrna9s7E_r4I-gbsemItA</recordid><startdate>20120127</startdate><enddate>20120127</enddate><creator>Figueroa-González, Gabriela</creator><creator>Jacobo-Herrera, Nadia</creator><creator>Zentella-Dehesa, Alejandro</creator><creator>Pereda-Miranda, Rogelio</creator><general>American Chemical Society and American Society of Pharmacognosy</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120127</creationdate><title>Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells</title><author>Figueroa-González, Gabriela ; Jacobo-Herrera, Nadia ; Zentella-Dehesa, Alejandro ; Pereda-Miranda, Rogelio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a413t-2a40220a6873ca61f166cc5175ae464aee169c12afb65d13131ac25bc00997633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antineoplastic Agents, Phytogenic - chemistry</topic><topic>Antineoplastic Agents, Phytogenic - isolation &amp; purification</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</topic><topic>Drug Resistance, Multiple - drug effects</topic><topic>Drug Resistance, Neoplasm - drug effects</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Female</topic><topic>Glycosides - chemistry</topic><topic>Glycosides - isolation &amp; purification</topic><topic>Glycosides - pharmacology</topic><topic>Humans</topic><topic>Ipomoea nil - chemistry</topic><topic>Molecular Structure</topic><topic>Rhodamines</topic><topic>Vinblastine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Figueroa-González, Gabriela</creatorcontrib><creatorcontrib>Jacobo-Herrera, Nadia</creatorcontrib><creatorcontrib>Zentella-Dehesa, Alejandro</creatorcontrib><creatorcontrib>Pereda-Miranda, Rogelio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural products (Washington, D.C.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Figueroa-González, Gabriela</au><au>Jacobo-Herrera, Nadia</au><au>Zentella-Dehesa, Alejandro</au><au>Pereda-Miranda, Rogelio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells</atitle><jtitle>Journal of natural products (Washington, D.C.)</jtitle><addtitle>J. Nat. Prod</addtitle><date>2012-01-27</date><risdate>2012</risdate><volume>75</volume><issue>1</issue><spage>93</spage><epage>97</epage><pages>93-97</pages><issn>0163-3864</issn><eissn>1520-6025</eissn><abstract>Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy.</abstract><cop>United States</cop><pub>American Chemical Society and American Society of Pharmacognosy</pub><pmid>22148475</pmid><doi>10.1021/np200864m</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0163-3864
ispartof Journal of natural products (Washington, D.C.), 2012-01, Vol.75 (1), p.93-97
issn 0163-3864
1520-6025
language eng
recordid cdi_proquest_miscellaneous_918579235
source MEDLINE; American Chemical Society Journals
subjects Antineoplastic Agents, Phytogenic - chemistry
Antineoplastic Agents, Phytogenic - isolation & purification
Antineoplastic Agents, Phytogenic - pharmacology
ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism
Drug Resistance, Multiple - drug effects
Drug Resistance, Neoplasm - drug effects
Drug Screening Assays, Antitumor
Female
Glycosides - chemistry
Glycosides - isolation & purification
Glycosides - pharmacology
Humans
Ipomoea nil - chemistry
Molecular Structure
Rhodamines
Vinblastine - pharmacology
title Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-18T19%3A41%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Reversal%20of%20Multidrug%20Resistance%20by%20Morning%20Glory%20Resin%20Glycosides%20in%20Human%20Breast%20Cancer%20Cells&rft.jtitle=Journal%20of%20natural%20products%20(Washington,%20D.C.)&rft.au=Figueroa-Gonza%CC%81lez,%20Gabriela&rft.date=2012-01-27&rft.volume=75&rft.issue=1&rft.spage=93&rft.epage=97&rft.pages=93-97&rft.issn=0163-3864&rft.eissn=1520-6025&rft_id=info:doi/10.1021/np200864m&rft_dat=%3Cproquest_cross%3E918579235%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1023041456&rft_id=info:pmid/22148475&rfr_iscdi=true