Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells
Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were...
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Veröffentlicht in: | Journal of natural products (Washington, D.C.) D.C.), 2012-01, Vol.75 (1), p.93-97 |
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creator | Figueroa-González, Gabriela Jacobo-Herrera, Nadia Zentella-Dehesa, Alejandro Pereda-Miranda, Rogelio |
description | Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy. |
doi_str_mv | 10.1021/np200864m |
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The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy.</description><identifier>ISSN: 0163-3864</identifier><identifier>EISSN: 1520-6025</identifier><identifier>DOI: 10.1021/np200864m</identifier><identifier>PMID: 22148475</identifier><language>eng</language><publisher>United States: American Chemical Society and American Society of Pharmacognosy</publisher><subject>Antineoplastic Agents, Phytogenic - chemistry ; Antineoplastic Agents, Phytogenic - isolation & purification ; Antineoplastic Agents, Phytogenic - pharmacology ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; Drug Resistance, Multiple - drug effects ; Drug Resistance, Neoplasm - drug effects ; Drug Screening Assays, Antitumor ; Female ; Glycosides - chemistry ; Glycosides - isolation & purification ; Glycosides - pharmacology ; Humans ; Ipomoea nil - chemistry ; Molecular Structure ; Rhodamines ; Vinblastine - pharmacology</subject><ispartof>Journal of natural products (Washington, D.C.), 2012-01, Vol.75 (1), p.93-97</ispartof><rights>Copyright © 2011 American Chemical Society and American Society of Pharmacognosy</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a413t-2a40220a6873ca61f166cc5175ae464aee169c12afb65d13131ac25bc00997633</citedby><cites>FETCH-LOGICAL-a413t-2a40220a6873ca61f166cc5175ae464aee169c12afb65d13131ac25bc00997633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/np200864m$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/np200864m$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22148475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Figueroa-González, Gabriela</creatorcontrib><creatorcontrib>Jacobo-Herrera, Nadia</creatorcontrib><creatorcontrib>Zentella-Dehesa, Alejandro</creatorcontrib><creatorcontrib>Pereda-Miranda, Rogelio</creatorcontrib><title>Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells</title><title>Journal of natural products (Washington, D.C.)</title><addtitle>J. Nat. Prod</addtitle><description>Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy.</description><subject>Antineoplastic Agents, Phytogenic - chemistry</subject><subject>Antineoplastic Agents, Phytogenic - isolation & purification</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>Drug Resistance, Multiple - drug effects</subject><subject>Drug Resistance, Neoplasm - drug effects</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Female</subject><subject>Glycosides - chemistry</subject><subject>Glycosides - isolation & purification</subject><subject>Glycosides - pharmacology</subject><subject>Humans</subject><subject>Ipomoea nil - chemistry</subject><subject>Molecular Structure</subject><subject>Rhodamines</subject><subject>Vinblastine - pharmacology</subject><issn>0163-3864</issn><issn>1520-6025</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90E1LwzAcBvAgipvTg19AchH1UM1726MW3YQNYei5pGk6OtpkJq3Qb2_m5k4iOSTh_8tDeAC4xOgeI4IfzIYglAjWHoEx5gRFAhF-DMYICxrRMBiBM-_XCCGKUn4KRoRglrCYj0G-1F_aedlAW8FF33R16foVXGpf-04apWExwIV1pjYrOG2sG35mJpwHZX1dag_Dbda30sAnp6XvYLZ952Cmm8afg5NKNl5f7PcJ-Hh5fs9m0fxt-po9ziPJMO0iIhkiBEmRxFRJgSsshFIcx1xqJpjUGotUYSKrQvAS07CkIrxQCKVpLCidgJtd7sbZz177Lm9rr8IPpNG293mKEx6nhPIgb_-VoVGKGGZcBHq3o8pZ752u8o2rW-mGgLYO54fmg73ax_ZFq8uD_K06gOsdkMrna9s7E_r4I-gbsemItA</recordid><startdate>20120127</startdate><enddate>20120127</enddate><creator>Figueroa-González, Gabriela</creator><creator>Jacobo-Herrera, Nadia</creator><creator>Zentella-Dehesa, Alejandro</creator><creator>Pereda-Miranda, Rogelio</creator><general>American Chemical Society and American Society of Pharmacognosy</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120127</creationdate><title>Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells</title><author>Figueroa-González, Gabriela ; Jacobo-Herrera, Nadia ; Zentella-Dehesa, Alejandro ; Pereda-Miranda, Rogelio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a413t-2a40220a6873ca61f166cc5175ae464aee169c12afb65d13131ac25bc00997633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antineoplastic Agents, Phytogenic - chemistry</topic><topic>Antineoplastic Agents, Phytogenic - isolation & purification</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</topic><topic>Drug Resistance, Multiple - drug effects</topic><topic>Drug Resistance, Neoplasm - drug effects</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Female</topic><topic>Glycosides - chemistry</topic><topic>Glycosides - isolation & purification</topic><topic>Glycosides - pharmacology</topic><topic>Humans</topic><topic>Ipomoea nil - chemistry</topic><topic>Molecular Structure</topic><topic>Rhodamines</topic><topic>Vinblastine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Figueroa-González, Gabriela</creatorcontrib><creatorcontrib>Jacobo-Herrera, Nadia</creatorcontrib><creatorcontrib>Zentella-Dehesa, Alejandro</creatorcontrib><creatorcontrib>Pereda-Miranda, Rogelio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural products (Washington, D.C.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Figueroa-González, Gabriela</au><au>Jacobo-Herrera, Nadia</au><au>Zentella-Dehesa, Alejandro</au><au>Pereda-Miranda, Rogelio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells</atitle><jtitle>Journal of natural products (Washington, D.C.)</jtitle><addtitle>J. Nat. Prod</addtitle><date>2012-01-27</date><risdate>2012</risdate><volume>75</volume><issue>1</issue><spage>93</spage><epage>97</epage><pages>93-97</pages><issn>0163-3864</issn><eissn>1520-6025</eissn><abstract>Reversal of multidrug resistance (MDR) by thirty resin glycosides from the morning glory family (Convolvulaceae) was evaluated in vinblastine-resistant human breast carcinoma cells (MCF-7/Vin). The effects of these amphipathic compounds on the cytotoxicity and P-glycoprotein (P-gp)-mediated MDR were estimated with the sulforhodamine B colorimetric assay. Active noncytotoxic compounds exerted a potentiation effect of vinblastine susceptibility by 1- to over 1906-fold at tested concentrations of 5 and 25 μg/mL. Murucoidin V (1) enhanced vinblastine activity 255-fold when incorporated at 25 μg/mL and also, based on flow cytometry, significantly increased the intracellular accumulation of rhodamine 123 with the use of reserpine as a positive control for a MDR reversal agent. Incubation of MCF-7/Vin cells with 1 caused an increase in uptake and notably lowered the efflux rate of rhodamine 123. Decreased expression of P-glycoprotein by compound 1 was detected by immunofluorescence flow cytometry after incubation with an anti-P-gp monoclonal antibody. These results suggest that resin glycosides represent potential efflux pump inhibitors for overcoming MDR in cancer therapy.</abstract><cop>United States</cop><pub>American Chemical Society and American Society of Pharmacognosy</pub><pmid>22148475</pmid><doi>10.1021/np200864m</doi><tpages>5</tpages></addata></record> |
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subjects | Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - isolation & purification Antineoplastic Agents, Phytogenic - pharmacology ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Drug Resistance, Multiple - drug effects Drug Resistance, Neoplasm - drug effects Drug Screening Assays, Antitumor Female Glycosides - chemistry Glycosides - isolation & purification Glycosides - pharmacology Humans Ipomoea nil - chemistry Molecular Structure Rhodamines Vinblastine - pharmacology |
title | Reversal of Multidrug Resistance by Morning Glory Resin Glycosides in Human Breast Cancer Cells |
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