Intracellular detection of interleukin 17 and other cytokines in human bronchoalveolar lavage fluid: A first assessment

Highlights ► Cytokines in human lung cells as candidate markers of disease are investigated. ► Cells are incubated with Brefeldin A with and without stimuli then stained for flow cytometry. ► Presence of IL-17 cells in unstimulated lymphocytes is one promising candidate. ► Percentages of IL-4 and -1...

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Veröffentlicht in:	Immunology letters 2012-01, Vol.141 (2), p.204-209
Hauptverfasser:	De Luca, Anna, Rindi, Laura, Celi, Alessandro, Melosini, Lorenza, Paggiaro, Pierluigi, Ceccherini Nelli, Luca, Garzelli, Carlo, Freer, Giulia
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Allergy and Immunology Alveoli Biomarkers - metabolism Bronchoalveolar Lavage Fluid - cytology Bronchoalveolar Lavage Fluid - immunology Bronchus CD4 antigen CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism CD4-Positive T-Lymphocytes - pathology Cytokines - metabolism Female Flow cytometry gamma -Interferon Granulocytes - immunology Granulocytes - metabolism Granulocytes - pathology Helper cells Histocompatibility antigen HLA Hospitals Humans Immunologic Memory Inflammation Interleukin 10 Interleukin 17 Interleukin 4 Interleukin-17 - analysis Interleukin-17 - metabolism Intracellular cytokine Intracellular Space Leukocytes (granulocytic) Lung Lung diseases Lung Diseases - diagnosis Lung Diseases - immunology Lung Diseases - pathology Lymphocytes T Macrophages Macrophages, Alveolar - immunology Macrophages, Alveolar - metabolism Macrophages, Alveolar - pathology Male Memory Middle Aged Peripheral blood Phorbol esters Th1-Th2 Balance Tumor necrosis factor Tumor necrosis factor- alpha
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Beschreibung
Zusammenfassung:	Highlights ► Cytokines in human lung cells as candidate markers of disease are investigated. ► Cells are incubated with Brefeldin A with and without stimuli then stained for flow cytometry. ► Presence of IL-17 cells in unstimulated lymphocytes is one promising candidate. ► Percentages of IL-4 and -10 in various cell subsets may also be interesting as markers of lung disease.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/j.imlet.2011.10.005