Rapid detection of blaIMP₋₆ by amplification refractory mutation system

Rapid detection of blaIMP₋₆ by amplification refractory mutation system

Klebsiella pneumoniae resistant to almost all ß-lactams except imipenem designated as ISMRK (imipenem-susceptible meropenem-resistant Klebsiella) is emerging in Japan. All ISMRK carries blaIMP₋₆ which differs from blaIMP₋₁ by only a single nucleotide at position 640. We devised a rapid detection sys...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of microbiological methods 2012, Vol.88 (1), p.182-184
Hauptverfasser: Kayama, Shizuo, Shigemoto, Norifumi, Kuwahara, Ryuichi, Onodera, Makoto, Yokozaki, Michiya, Ohge, Hiroki, Kato, Fuminori, Hisatsune, Jyunzo, Sugai, Motoyuki
Format: Artikel
Sprache:eng
Schlagworte:
Anti-Bacterial Agents - pharmacology
Bacterial Proteins - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriology
beta-Lactamases - genetics
Biological and medical sciences
Drug Resistance, Bacterial
Fundamental and applied biological sciences. Psychology
Humans
imipenem
Imipenem - pharmacology
Japan
Klebsiella
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Microbial Sensitivity Tests
Microbiology
Mutation
polymerase chain reaction
Polymerase Chain Reaction - methods
rapid methods
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Klebsiella pneumoniae resistant to almost all ß-lactams except imipenem designated as ISMRK (imipenem-susceptible meropenem-resistant Klebsiella) is emerging in Japan. All ISMRK carries blaIMP₋₆ which differs from blaIMP₋₁ by only a single nucleotide at position 640. We devised a rapid detection system of blaIMP₋₆ by using ARMS PCR.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2011.10.022