The nucleoporin Nup153 maintains nuclear envelope architecture and is required for cell migration in tumor cells

Nucleoporin 153 (Nup153), a component of the nuclear pore complex (NPC), has been implicated in the interaction of the NPC with the nuclear lamina. Here we show that depletion of Nup153 by RNAi results in alteration of the organization of the nuclear lamina and the nuclear lamin-binding protein Sun1...

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Veröffentlicht in:	FEBS letters 2010-07, Vol.584 (14), p.3013-3020
Hauptverfasser:	Zhou, Lixin, Panté, Nelly
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carrier Proteins - genetics Carrier Proteins - metabolism Cell migration Cellular Structures - metabolism classical nuclear localization sequence cNLS Cytoskeleton Cytoskeleton - genetics Cytoskeleton - metabolism EdU ethynyl deoxyuridine Humans Intermediate Filament Proteins - genetics Intermediate Filament Proteins - metabolism LINC linker of nucleoskeleton and cytoskeleton microtubule-organizing centre MTOC NPC nuclear envelope Nuclear Envelope - genetics Nuclear Envelope - metabolism Nuclear lamina Nuclear Lamina - genetics Nuclear Lamina - metabolism Nuclear Pore - genetics Nuclear Pore - metabolism Nuclear pore complex Nuclear Pore Complex Proteins - genetics Nuclear Pore Complex Proteins - metabolism Nuclear Proteins - genetics Nuclear Proteins - metabolism Nucleoporin Nucleoporin 153 Nup153 Protein Binding - genetics RNA Interference Xenopus laevis
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Zusammenfassung:	Nucleoporin 153 (Nup153), a component of the nuclear pore complex (NPC), has been implicated in the interaction of the NPC with the nuclear lamina. Here we show that depletion of Nup153 by RNAi results in alteration of the organization of the nuclear lamina and the nuclear lamin-binding protein Sun1. More striking, Nup153 depletion induces a dramatic cytoskeletal rearrangement that impairs cell migration in human breast carcinoma cells. Our results point to a very prominent role of Nup153 in connection to cell motility that could be exploited in order to develop novel anti-cancer therapy. MINT- 7893777: Lamin-A/C (uniprotkb: P02545) and NUP153 (uniprotkb: P49790) colocalize (MI: 0403) by fluorescence microscopy (MI: 0416) MINT- 7893761: sun1 (uniprotkb: Q9D666) and Lamin-A/C (uniprotkb: P02545) colocalize (MI: 0403) by fluorescence microscopy (MI: 0416)
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2010.05.038