Antisense oligodeoxynucleotide complexation with phosphoryl-choline diblock copolymers and its effect on cell transfection

MPC30-DEA70/c-myc AS-ODN complexes were formed at different N/P ratios and transferred into HeLa cells. The complexes were studied using gel electrophoresis, fluorescent microscopy, FCM, and MTT assay. It was shown that the novel cationic phosphorylcholine copolymer MPC30–DEA70 could load and transp...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of controlled release 2011-11, Vol.152, p.e172-e173
Hauptverfasser:	Sun, Tiantian, Zhang, Zhuoqi, Cao, Xichuan, Zhang, Min, Zhao, Xia, Zhang, Qi, Chen, Minmin
Format:	Artikel
Sprache:	eng
Schlagworte:	Antisense oligodeoxynucleotide Biological Transport cell growth Cell Proliferation Cell Survival - drug effects Cell transfection composite polymers Dose-Response Relationship, Drug Electrophoresis, Agar Gel Flow Cytometry fluorescence gel electrophoresis Gene delivery growth retardation HeLa Cells Humans microscopy Microscopy, Fluorescence oligodeoxyribonucleotides Oligodeoxyribonucleotides, Antisense - chemistry Oligodeoxyribonucleotides, Antisense - metabolism PC polymer Phosphorylcholine - analogs & derivatives Phosphorylcholine - chemistry Phosphorylcholine - toxicity Polymerization Polymers - chemistry Polymers - toxicity Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism transfection Transfection - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	MPC30-DEA70/c-myc AS-ODN complexes were formed at different N/P ratios and transferred into HeLa cells. The complexes were studied using gel electrophoresis, fluorescent microscopy, FCM, and MTT assay. It was shown that the novel cationic phosphorylcholine copolymer MPC30–DEA70 could load and transport human c-myc antisense oligonucleotides. The transfection efficiency of the complexes and cell growth inhibition were significantly enhanced with increasing of N/P ratios.
ISSN:	0168-3659 1873-4995
DOI:	10.1016/j.jconrel.2011.08.070