Human β-Defensins and Psoriasin/S100A7 Expression in Salivary Glands: Anti-Oncogenic Molecules for Potential Therapeutic Approaches
Background Host defence peptides (HDPs), including human β-defensins (hBDs) and psoriasin/S100A7, exert antimicrobial and immunoregulatory functions of the innate defense system. In addition to these functions, the search for cancer biomarkers has identified HDPs as playing a potential role in both...
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| Veröffentlicht in: | BioDrugs : clinical immunotherapeutics, biopharmaceuticals, and gene therapy biopharmaceuticals, and gene therapy, 2012-02, Vol.26 (1), p.33-42 |
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| container_title | BioDrugs : clinical immunotherapeutics, biopharmaceuticals, and gene therapy |
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| creator | Kesting, Marco R. Stoeckelhuber, Mechthild Kuppek, Alexandra Hasler, Rafael Rohleder, Nils Wolff, Klaus-Dietrich Nieberler, Markus |
| description | Background
Host defence peptides (HDPs), including human β-defensins (hBDs) and psoriasin/S100A7, exert antimicrobial and immunoregulatory functions of the innate defense system. In addition to these functions, the search for cancer biomarkers has identified HDPs as playing a potential role in both tumor suppression and oncogenesis. Although HDPs are highly expressed in salivary glands, their role as molecules for potential diagnostic and therapeutic approaches has not yet been analyzed.
Objective
The aim of the present study was to investigate whether expression levels of putative pro- or antioncogenic hBDs, including hBD-1, -2, -3, and psoriasin/S 100A7, are altered in salivary gland tumor tissue as potential targets for molecular-based therapeutic approaches.
Methods
We analyzed the expression levels of hBD-1, -2, -3, and psoriasin/S100A7 by quantitative real-time polymerase chain reaction (qrt-PCR) and immunohistochemistry in a case control study by comparing salivary gland tumor samples relative to healthy control specimens from 58 patients. Expression level analysis of hBD-1, -2, -3, and psoriasin/S 100A7 by qrt-PCR was normalized to the endogenous 18S rRNA expression levels.
Results
The results demonstrate the significant downregulation of hBD-1 (p |
| doi_str_mv | 10.2165/11597570-000000000-00000 |
| format | Article |
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Host defence peptides (HDPs), including human β-defensins (hBDs) and psoriasin/S100A7, exert antimicrobial and immunoregulatory functions of the innate defense system. In addition to these functions, the search for cancer biomarkers has identified HDPs as playing a potential role in both tumor suppression and oncogenesis. Although HDPs are highly expressed in salivary glands, their role as molecules for potential diagnostic and therapeutic approaches has not yet been analyzed.
Objective
The aim of the present study was to investigate whether expression levels of putative pro- or antioncogenic hBDs, including hBD-1, -2, -3, and psoriasin/S 100A7, are altered in salivary gland tumor tissue as potential targets for molecular-based therapeutic approaches.
Methods
We analyzed the expression levels of hBD-1, -2, -3, and psoriasin/S100A7 by quantitative real-time polymerase chain reaction (qrt-PCR) and immunohistochemistry in a case control study by comparing salivary gland tumor samples relative to healthy control specimens from 58 patients. Expression level analysis of hBD-1, -2, -3, and psoriasin/S 100A7 by qrt-PCR was normalized to the endogenous 18S rRNA expression levels.
Results
The results demonstrate the significant downregulation of hBD-1 (p<0.001), hBD-2 (p = 0.003), hBD-3 (p = 0.002), and psoriasin/S 100A7 (p = 0.003) mRNA in human salivary gland tumors compared with healthy control specimens. Protein expression levels of hBD-1, -2, -3, and psoriasin/S 100A7 in salivary gland tumor tissue were strongly reduced compared with healthy control specimens.
Conclusion
The data indicates a putative role of the innate defense system in salivary gland tumor formation. The identification of immunoregulatory molecules as diagnostic biomarkers or therapeutic targets could provide new approaches for molecular-based diagnostic and therapeutic support to treat salivary gland tumors as well as other malignancies. We suggest that HDPs should be taken into consideration for use in molecular-based therapeutic approaches.</description><identifier>ISSN: 1173-8804</identifier><identifier>EISSN: 1179-190X</identifier><identifier>DOI: 10.2165/11597570-000000000-00000</identifier><identifier>PMID: 22149099</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies ; beta-Defensins - biosynthesis ; Biomarkers, Tumor - biosynthesis ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Case-Control Studies ; Child ; Down-Regulation ; Female ; Humans ; Male ; Middle Aged ; Molecular Medicine ; Original Research Article ; Pharmacotherapy ; S100 Calcium Binding Protein A7 ; S100 Proteins - biosynthesis ; Salivary Gland Neoplasms - metabolism ; Salivary Glands - metabolism</subject><ispartof>BioDrugs : clinical immunotherapeutics, biopharmaceuticals, and gene therapy, 2012-02, Vol.26 (1), p.33-42</ispartof><rights>Adis Data Information BV 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c309t-1ed1ae948ae0cf3b5ed50fa5863b144a763af2c850580ff8c7504360c4a2f973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2165/11597570-000000000-00000$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2165/11597570-000000000-00000$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22149099$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kesting, Marco R.</creatorcontrib><creatorcontrib>Stoeckelhuber, Mechthild</creatorcontrib><creatorcontrib>Kuppek, Alexandra</creatorcontrib><creatorcontrib>Hasler, Rafael</creatorcontrib><creatorcontrib>Rohleder, Nils</creatorcontrib><creatorcontrib>Wolff, Klaus-Dietrich</creatorcontrib><creatorcontrib>Nieberler, Markus</creatorcontrib><title>Human β-Defensins and Psoriasin/S100A7 Expression in Salivary Glands: Anti-Oncogenic Molecules for Potential Therapeutic Approaches</title><title>BioDrugs : clinical immunotherapeutics, biopharmaceuticals, and gene therapy</title><addtitle>BioDrugs</addtitle><addtitle>BioDrugs</addtitle><description>Background
Host defence peptides (HDPs), including human β-defensins (hBDs) and psoriasin/S100A7, exert antimicrobial and immunoregulatory functions of the innate defense system. In addition to these functions, the search for cancer biomarkers has identified HDPs as playing a potential role in both tumor suppression and oncogenesis. Although HDPs are highly expressed in salivary glands, their role as molecules for potential diagnostic and therapeutic approaches has not yet been analyzed.
Objective
The aim of the present study was to investigate whether expression levels of putative pro- or antioncogenic hBDs, including hBD-1, -2, -3, and psoriasin/S 100A7, are altered in salivary gland tumor tissue as potential targets for molecular-based therapeutic approaches.
Methods
We analyzed the expression levels of hBD-1, -2, -3, and psoriasin/S100A7 by quantitative real-time polymerase chain reaction (qrt-PCR) and immunohistochemistry in a case control study by comparing salivary gland tumor samples relative to healthy control specimens from 58 patients. Expression level analysis of hBD-1, -2, -3, and psoriasin/S 100A7 by qrt-PCR was normalized to the endogenous 18S rRNA expression levels.
Results
The results demonstrate the significant downregulation of hBD-1 (p<0.001), hBD-2 (p = 0.003), hBD-3 (p = 0.002), and psoriasin/S 100A7 (p = 0.003) mRNA in human salivary gland tumors compared with healthy control specimens. Protein expression levels of hBD-1, -2, -3, and psoriasin/S 100A7 in salivary gland tumor tissue were strongly reduced compared with healthy control specimens.
Conclusion
The data indicates a putative role of the innate defense system in salivary gland tumor formation. The identification of immunoregulatory molecules as diagnostic biomarkers or therapeutic targets could provide new approaches for molecular-based diagnostic and therapeutic support to treat salivary gland tumors as well as other malignancies. We suggest that HDPs should be taken into consideration for use in molecular-based therapeutic approaches.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Antibodies</subject><subject>beta-Defensins - biosynthesis</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Case-Control Studies</subject><subject>Child</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Medicine</subject><subject>Original Research Article</subject><subject>Pharmacotherapy</subject><subject>S100 Calcium Binding Protein A7</subject><subject>S100 Proteins - biosynthesis</subject><subject>Salivary Gland Neoplasms - metabolism</subject><subject>Salivary Glands - metabolism</subject><issn>1173-8804</issn><issn>1179-190X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFOwzAQRS0EoqVwBeQdq9BxHMf2siqlRaoEUrtgZ7mJjVIlTrEbBNfiIJwJQ2i3eOM_8ps_448QJnCbkpyNCWGSMw4JHE6vTtCQEC4TIuH59FfTRAjIBugihG0Ecir5ORqkKckkSDlEs0XXaIe_PpM7Y40LlQtYuxI_hdZXOpbjFQGYcDx733kTQtU6XDm80nX1pv0HnteRDpfozOo6mKu_e4TW97P1dJEsH-cP08kyKSjIfUJMSbSRmdAGCks3zJQMrGYipxuSZZrnVNu0EAyYAGtFwRlkNIci06mVnI7QTW-78-1rZ8JeNVUoTB13MG0XlCQsp4TKNJKiJwvfhuCNVTtfNXFhRUD9RKgOEapjhL2Krdd_Q7pNY8pj4yGzCMgeCPHJvRivtm3nXfz3_-bfQCZ7vA</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Kesting, Marco R.</creator><creator>Stoeckelhuber, Mechthild</creator><creator>Kuppek, Alexandra</creator><creator>Hasler, Rafael</creator><creator>Rohleder, Nils</creator><creator>Wolff, Klaus-Dietrich</creator><creator>Nieberler, Markus</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120201</creationdate><title>Human β-Defensins and Psoriasin/S100A7 Expression in Salivary Glands</title><author>Kesting, Marco R. ; Stoeckelhuber, Mechthild ; Kuppek, Alexandra ; Hasler, Rafael ; Rohleder, Nils ; Wolff, Klaus-Dietrich ; Nieberler, Markus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c309t-1ed1ae948ae0cf3b5ed50fa5863b144a763af2c850580ff8c7504360c4a2f973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Antibodies</topic><topic>beta-Defensins - biosynthesis</topic><topic>Biomarkers, Tumor - biosynthesis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Case-Control Studies</topic><topic>Child</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Medicine</topic><topic>Original Research Article</topic><topic>Pharmacotherapy</topic><topic>S100 Calcium Binding Protein A7</topic><topic>S100 Proteins - biosynthesis</topic><topic>Salivary Gland Neoplasms - metabolism</topic><topic>Salivary Glands - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kesting, Marco R.</creatorcontrib><creatorcontrib>Stoeckelhuber, Mechthild</creatorcontrib><creatorcontrib>Kuppek, Alexandra</creatorcontrib><creatorcontrib>Hasler, Rafael</creatorcontrib><creatorcontrib>Rohleder, Nils</creatorcontrib><creatorcontrib>Wolff, Klaus-Dietrich</creatorcontrib><creatorcontrib>Nieberler, Markus</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>BioDrugs : clinical immunotherapeutics, biopharmaceuticals, and gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kesting, Marco R.</au><au>Stoeckelhuber, Mechthild</au><au>Kuppek, Alexandra</au><au>Hasler, Rafael</au><au>Rohleder, Nils</au><au>Wolff, Klaus-Dietrich</au><au>Nieberler, Markus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human β-Defensins and Psoriasin/S100A7 Expression in Salivary Glands: Anti-Oncogenic Molecules for Potential Therapeutic Approaches</atitle><jtitle>BioDrugs : clinical immunotherapeutics, biopharmaceuticals, and gene therapy</jtitle><stitle>BioDrugs</stitle><addtitle>BioDrugs</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>26</volume><issue>1</issue><spage>33</spage><epage>42</epage><pages>33-42</pages><issn>1173-8804</issn><eissn>1179-190X</eissn><abstract>Background
Host defence peptides (HDPs), including human β-defensins (hBDs) and psoriasin/S100A7, exert antimicrobial and immunoregulatory functions of the innate defense system. In addition to these functions, the search for cancer biomarkers has identified HDPs as playing a potential role in both tumor suppression and oncogenesis. Although HDPs are highly expressed in salivary glands, their role as molecules for potential diagnostic and therapeutic approaches has not yet been analyzed.
Objective
The aim of the present study was to investigate whether expression levels of putative pro- or antioncogenic hBDs, including hBD-1, -2, -3, and psoriasin/S 100A7, are altered in salivary gland tumor tissue as potential targets for molecular-based therapeutic approaches.
Methods
We analyzed the expression levels of hBD-1, -2, -3, and psoriasin/S100A7 by quantitative real-time polymerase chain reaction (qrt-PCR) and immunohistochemistry in a case control study by comparing salivary gland tumor samples relative to healthy control specimens from 58 patients. Expression level analysis of hBD-1, -2, -3, and psoriasin/S 100A7 by qrt-PCR was normalized to the endogenous 18S rRNA expression levels.
Results
The results demonstrate the significant downregulation of hBD-1 (p<0.001), hBD-2 (p = 0.003), hBD-3 (p = 0.002), and psoriasin/S 100A7 (p = 0.003) mRNA in human salivary gland tumors compared with healthy control specimens. Protein expression levels of hBD-1, -2, -3, and psoriasin/S 100A7 in salivary gland tumor tissue were strongly reduced compared with healthy control specimens.
Conclusion
The data indicates a putative role of the innate defense system in salivary gland tumor formation. The identification of immunoregulatory molecules as diagnostic biomarkers or therapeutic targets could provide new approaches for molecular-based diagnostic and therapeutic support to treat salivary gland tumors as well as other malignancies. We suggest that HDPs should be taken into consideration for use in molecular-based therapeutic approaches.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>22149099</pmid><doi>10.2165/11597570-000000000-00000</doi><tpages>10</tpages></addata></record> |
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| ispartof | BioDrugs : clinical immunotherapeutics, biopharmaceuticals, and gene therapy, 2012-02, Vol.26 (1), p.33-42 |
| issn | 1173-8804 1179-190X |
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| subjects | Adolescent Adult Aged Aged, 80 and over Antibodies beta-Defensins - biosynthesis Biomarkers, Tumor - biosynthesis Biomedical and Life Sciences Biomedicine Cancer Research Case-Control Studies Child Down-Regulation Female Humans Male Middle Aged Molecular Medicine Original Research Article Pharmacotherapy S100 Calcium Binding Protein A7 S100 Proteins - biosynthesis Salivary Gland Neoplasms - metabolism Salivary Glands - metabolism |
| title | Human β-Defensins and Psoriasin/S100A7 Expression in Salivary Glands: Anti-Oncogenic Molecules for Potential Therapeutic Approaches |
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