Human β-Defensins and Psoriasin/S100A7 Expression in Salivary Glands: Anti-Oncogenic Molecules for Potential Therapeutic Approaches

Background Host defence peptides (HDPs), including human β-defensins (hBDs) and psoriasin/S100A7, exert antimicrobial and immunoregulatory functions of the innate defense system. In addition to these functions, the search for cancer biomarkers has identified HDPs as playing a potential role in both tumor suppression and oncogenesis. Although HDPs are highly expressed in salivary glands, their role as molecules for potential diagnostic and therapeutic approaches has not yet been analyzed. Objective The aim of the present study was to investigate whether expression levels of putative pro- or antioncogenic hBDs, including hBD-1, -2, -3, and psoriasin/S 100A7, are altered in salivary gland tumor tissue as potential targets for molecular-based therapeutic approaches. Methods We analyzed the expression levels of hBD-1, -2, -3, and psoriasin/S100A7 by quantitative real-time polymerase chain reaction (qrt-PCR) and immunohistochemistry in a case control study by comparing salivary gland tumor samples relative to healthy control specimens from 58 patients. Expression level analysis of hBD-1, -2, -3, and psoriasin/S 100A7 by qrt-PCR was normalized to the endogenous 18S rRNA expression levels. Results The results demonstrate the significant downregulation of hBD-1 (p