Evolution in the sensitivity of quantitative HIV-1 viral load tests

Abstract Significant advancements in molecular diagnostics have been made since the inception and application of PCR-based technologies in clinical diagnostic laboratories and the management of HIV-1 infected patients. More recently, real-time PCR has improved the overall performance of assays used...

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Veröffentlicht in:	Journal of clinical virology 2011-12, Vol.52, p.S77-S82
Hauptverfasser:	Cobb, Bryan R, Vaks, Jeffrey E, Do, Tri, Vilchez, Regis A
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Guidelines as Topic - standards HIV Infections - diagnosis HIV Infections - immunology HIV Infections - virology HIV viral load HIV-1 HIV-1 - genetics HIV-1 - immunology HIV-1 - pathogenicity Human immunodeficiency virus 1 Humans Infectious Disease Lower limit of detection Lower limit of quantification Real-time PCR Real-Time Polymerase Chain Reaction - standards RNA, Viral - analysis Sensitivity and Specificity Viral Load - immunology Viral Load - methods
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Beschreibung
Zusammenfassung:	Abstract Significant advancements in molecular diagnostics have been made since the inception and application of PCR-based technologies in clinical diagnostic laboratories and the management of HIV-1 infected patients. More recently, real-time PCR has improved the overall performance of assays used for detecting and quantifying HIV-1 RNA viral load in patients undergoing antiretroviral treatment. The effects of these changes and the interpretations of the HIV-1 viral load results are discussed in this review in the context of the different assays used, the viral dynamics of the HIV-1 virus, and the recent changes to HIV-1 treatment guidelines.
ISSN:	1386-6532 1873-5967
DOI:	10.1016/j.jcv.2011.09.015