Absorption optical density as a diagnostic tool for indicating the toxicity of gold nanoparticles

Nanotechnology has recently emerged as a promising approach for treatment and diagnosis of a variety of diseases. The aim of this study was to evaluate the blood absorption spectra to assess bioaccumulation and toxic effects of 100 mu l of 10 and 50 nm gold nanoparticles (GNPs) upon intraperitoneal administration in rats for periods of three and seven days. Healthy 30 male Wistar-Kyoto rats were individually caged and divided into control group (NG: n = 10), group 1 (A: infusion of 10 nm GNPs for three days; n = 5; B: infusion of 10 nm GNPs for seven days; n = 5) and group 2 (A: infusion of 50 nm GNPs for three days; n = 5; B: infusion of 50 nm GNPs for seven days; n = 5). Dose of 100 mu l of 10 and 50 nm GNPs were administered intraperitonealy to the animals. The blood absorbance peaks for G1A, G1B, G2A and G2B significantly decreased compared with the control. The blood absorbance peaks for G1A significantly decreased compared with G1B; and G2A significantly decreased compared with G2B. This implies that G1A is highly reactive than G1B, and G2A is highly reactive than G2B, A significant decrease (p