Expansion of the Amino Acid Repertoire in Protein Biosynthesis in Silkworm Cells

Expansion of the Amino Acid Repertoire in Protein Biosynthesis in Silkworm Cells

Toward silk production with UAAs: The strategy for residue‐specific incorporation of unnatural amino acids (UAAs) into proteins was extended to silkworm cells. Here we report the incorporation of p‐Cl‐Phe into a reporter protein (EGFP) synthesized in silkworm cells that express an αA450G mutant of B...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2012-01, Vol.13 (1), p.61-65
Hauptverfasser: Teramoto, Hidetoshi, Kojima, Katsura, Kajiwara, Hideyuki, Ishibashi, Jun
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
amino acids
aminoacyl-tRNA synthetases
Animals
Bombyx - cytology
Bombyx - metabolism
Cells, Cultured
Insect Proteins - biosynthesis
Insect Proteins - chemistry
Insect Proteins - genetics
Phenylalanine - chemistry
Phenylalanine - genetics
Protein Biosynthesis
protein engineering
silkworms
unnatural amino acids
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Zusammenfassung:Toward silk production with UAAs: The strategy for residue‐specific incorporation of unnatural amino acids (UAAs) into proteins was extended to silkworm cells. Here we report the incorporation of p‐Cl‐Phe into a reporter protein (EGFP) synthesized in silkworm cells that express an αA450G mutant of B. mori phenylalanyl‐tRNA synthetase with relaxed substrate specificity.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201100624