Effect of bleaching on tooth discolouration from food colourant in vitro

Abstract Objectives The purpose of this study was to evaluate the effect of bleaching on tooth discolouration from neutral food colourant media (NFCM). Methods Freshly extracted human molar teeth ( n = 32) were divided into four groups ( n = 8): non-bleached treatment-NBT, non-bleached control-NBC, bleached treatment-BT and bleached control-BC. Bleached teeth specimens were subjected to 20% carbamide peroxide bleaching agent for 10 h. Colour measurements (pre-test) were done using a non-contact spectroradiometer. The teeth specimens were immersed in a neutral buffer solution. NBT and BT groups had 0.025% red food colour (Red 40) added to the buffer solution. The immersed specimens were incubated for 4 h at 37 °C. Colour was again measured after immersion/incubation (post-test) and after subsequent polishing using plain-pumice slurry (post-polish). Differences in CIE a* (redness parameter) were analysed by repeated measures ANOVA with Bonferroni-corrected t -tests for the pairwise comparisons of interest. Results Tooth discolouration was measured as total stain (mean difference in a* between post-test minus pre-test), extrinsic stain (mean difference in a* post-polish minus post-test) and intrinsic stain (mean difference in a* post-polish minus pre-test) A statistically significant difference in the mean Δ a* was observed ( P = 0.009) between staining treatment for bleached and non-bleached teeth. Also, a statistically significant difference ( P < 0.001) was noted between control and treatment groups of bleached teeth that were subjected to staining. Conclusions Bleaching resulted in total and extrinsic tooth discolouration by the NFCM. Clinical significance It might be beneficial to avoid highly pigmented foods immediately following bleaching in order to optimize the effects of tooth whitening.