BACTEC MGIT 960 system for screening of Mycobacterium tuberculosis complex among cattle

Bovine tuberculosis remains a disease of economic and public health importance in developing countries. The largest number of new cases of tuberculosis usually occurs in South-East Asia region and Africa. This study was aimed to evaluate the recent technique (BACTEC MGIT 960 system) for screening of Mycobacterium tuberculosis complex among cattle in Egypt. From the 1180 cattle examined in three different Governorates (El-Sharkia, El-Gharbia and El-Monefeia) by single intradermal tuberculin test, 29 animals (2.46%) were positive reactors. The post mortem examination of the positive reactors showed that 22 animals (75.9%) had visible lesions [respiratory form (31.0%), digestive form (13.8%), mixed form (20.7%) and generalized form (10.3%)], while seven (24.1%) did not show visible lesions. The results of isolation and identification using conventional culture method (Lowenstein-Jensen medium) were 22 mycobacterial isolates (75.9%), 20 (68.97%) Mycobacterium bovis and 2 (6.9%) unidentified slow growth. The BACTEC MGIT 960 system was used for recovery of Mycobacteria and compared with conventional culture method (Lowenstein-Jensen medium). The recovery rate of BACTEC MGIT 960 system was 82.8%, while that of Lowenstein-Jensen medium was 75.9%. The mean time for detection of Mycobacteria was 17.8 0.9 days and 46.5 plus or minus 0.4 days for BACTEC MGIT 960 system and Lowenstein-Jensen medium, respectively while the contamination rate with BACTEC MGIT 960 system was 6.9% and 10.3% in Lowenstein-Jensen medium.