Antimicrobial activity of Crataeva religiosa Forst against bacteria isolated from Thryonomys swinderianus Temminck

An attempt has been made to carry out a screening on the antibacterial activity of leaves of Crateva religiosa Forst used in Benin traditional veterinary medicine against bacterial infection of Thryonomys swinderianus (class of Mammalia, family of Thryonomyidae) commonly called agouti or kholan. The aim of this study was to select the most active extracts and fractions which may be useful to combat these bacterial infections. Seven extracts from C. religiosa were screened for their antibacterial. The antibacterial activity was evaluated by both microtest method using p-iodonitrotetrazolium and bioautography against five microorganisms obtained from T. swinderianus (Escherichia coli, Shigella sonei, Staphylococcus aureus, Pasteurela pestis and Yersinia enterocolitica). The minimum inhibitory concentration (MIC) and the total activity (TA) were determined. All extracts were effective against tested microorganisms at different levels (0.31 less than or equal to MIC less than or equal to 10 mg/ml). The screening experiment revealed that ethyl acetate extract was more potent than other extracts with the MIC values of 0.62 mg/ml against E. coli and 0.31 mg/ml against S. aureus, S. sonei, P. pestis and Y. enterocolitica. The results provide an evidence for the traditional use of C. religiosa for the treatment of infective diseases of T. swindehanus Temminck.