Objective assessment of the cryoprotective effects of dimethylformamide for freezing goat semen

The aim of this work was to assess the cryoprotective effects of dimethylformamide (DMF) for freezing goat semen, using an objective analysis by computer-assisted sperm analysis (CASA). Twenty-one ejaculates (seven per animal) were collected from three stud bucks with the aid of an artificial vagina...

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Veröffentlicht in:	Cryobiology 2011-12, Vol.63 (3), p.263-266
Hauptverfasser:	Bezerra, Francisco Silvestre B., Castelo, Thibério S., Alves, Heron M., Oliveira, Isabelle R.S., Lima, Gabriela L., Peixoto, Gislayne C.X., Bezerra, Ana Carla S.D., Silva, Alexandre R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Survival - drug effects Cryopreservation - methods Cryoprotectant Cryoprotective Agents - pharmacology Dimethylformamide Dimethylformamide - pharmacology Egg Yolk Excipients - pharmacology Freezing Fundamental and applied biological sciences. Psychology Glycerol Glycerol - pharmacology Goat Goats Male Mammalian reproduction. General aspects Microscopy, Phase-Contrast Semen - drug effects Semen - physiology Semen Analysis Semen Preservation - methods Sperm Sperm Motility - drug effects Vertebrates: reproduction
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Zusammenfassung:	The aim of this work was to assess the cryoprotective effects of dimethylformamide (DMF) for freezing goat semen, using an objective analysis by computer-assisted sperm analysis (CASA). Twenty-one ejaculates (seven per animal) were collected from three stud bucks with the aid of an artificial vagina and immediately evaluated for gross and microscopic characteristics. The semen was diluted in two steps with a Tris–egg yolk extender containing 6% glycerol or 6% DMF, frozen in 0.50-mL straws, and stored in liquid nitrogen. Samples were accessed for sperm morphology, sperm membrane structural and functional integrity, and by CASA, immediately after thawing. There were differences (P
ISSN:	0011-2240 1090-2392
DOI:	10.1016/j.cryobiol.2011.09.136