Molecular structure and characterization of the cytokine TWEAK and its receptor Fn14 in bovine

Full-length cDNA encoding TWEAK and Fn14 from bovine was isolated. We used bioinformatics to analyze the gene structure, function, evolutionary relationships, and predicted three-dimensional structure of proteins and binding sites. Real-time quantitative PCR (Q-PCR) analysis revealed that both TWEAK...

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Veröffentlicht in:	Veterinary immunology and immunopathology 2011-12, Vol.144 (3), p.238-246
Hauptverfasser:	Zhang, Jia-Xin, Sang, Ming, Li, Jian-Feng, Zhao, Wei, Ma, Hong-Wei, Min, Cui, Hu, Yun-Long, Du, Ming-Xiang, Zhang, Shuang-Quan
Format:	Artikel
Sprache:	eng
Schlagworte:	3D structure and binding sites Amino Acid Sequence Animals Base Sequence Binding Sites Bioinformatics analyses Cattle Molecular Sequence Data Phylogeny Protein Conformation Real-Time Polymerase Chain Reaction Real-time Q-PCR Receptors, Tumor Necrosis Factor - chemistry Receptors, Tumor Necrosis Factor - genetics Receptors, Tumor Necrosis Factor - metabolism The TNF ligand/receptor interaction Tissue Distribution Tumor Necrosis Factors - chemistry Tumor Necrosis Factors - genetics Tumor Necrosis Factors - metabolism TWEAK and Fn14 TWEAK Receptor Two-Hybrid System Techniques
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Zusammenfassung:	Full-length cDNA encoding TWEAK and Fn14 from bovine was isolated. We used bioinformatics to analyze the gene structure, function, evolutionary relationships, and predicted three-dimensional structure of proteins and binding sites. Real-time quantitative PCR (Q-PCR) analysis revealed that both TWEAK and Fn14 are constitutively expressed in various tissues in bovine. Bovine TWEAK and Fn14 interaction analysis by yeast two-hybrid. Our results suggest that the TWEAK–Fn14 pathway is evolutionarily highly conserved. It will be helpful for investigation on the biological role of the TWEAK/Fn14 system in this important animal model. Furthermore, it provides insight into the molecular evolution of the emerging TWEAK and Fn14 families.
ISSN:	0165-2427 1873-2534
DOI:	10.1016/j.vetimm.2011.09.002