Effect of probiotic fermented milk and chlorophyllin on gene expressions and genotoxicity during AFB₁-induced hepatocellular carcinoma

Effect of probiotic fermented milk and chlorophyllin on gene expressions and genotoxicity during AFB₁-induced hepatocellular carcinoma

The aim of this study was to investigate the chemopreventive effect of probiotic fermented milk and chlorophyllin on aflatoxin B₁ (AFB₁) induced hepatocellular carcinoma. In vivo trials were conducted on 200 Wistar rats allocated to eight groups. Rats in the positive control group were given intrape...

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Veröffentlicht in:Gene 2011-12, Vol.490 (1-2), p.54-59
Hauptverfasser: Kumar, Manoj, Verma, Vinod, Nagpal, Ravinder, Kumar, Ashok, Gautam, Sanjeev K, Behare, Pradip V, Grover, Chand R, Aggarwal, Praveen K
Format: Artikel
Sprache:eng
Schlagworte:
Aflatoxin B1
Animals
body weight
carcinogenesis
Carcinoma, Hepatocellular - chemically induced
Carcinoma, Hepatocellular - prevention & control
chemoprevention
Chlorophyllides - pharmacology
Comet Assay
Cultured Milk Products
DNA Damage
fermented milk
Gene Expression
genes
genotoxicity
hepatoma
intraperitoneal injection
Liver Neoplasms - chemically induced
Liver Neoplasms - prevention & control
Male
Mutagens
probiotics
Probiotics - pharmacology
Rats
Rats, Wistar
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Zusammenfassung:The aim of this study was to investigate the chemopreventive effect of probiotic fermented milk and chlorophyllin on aflatoxin B₁ (AFB₁) induced hepatocellular carcinoma. In vivo trials were conducted on 200 Wistar rats allocated to eight groups. Rats in the positive control group were given intraperitoneal injection of aflatoxin B₁ at 450μg/kg body weight twice a week for 6weeks. The rats were sacrificed and dissected at 25th week of the experiment, and comet assay was carried out in hepatic cells to assess the genotoxicity or DNA damage. The tumour incidence was decreased by approximately one-third than AFB₁ control group. The expression of c-myc bax, bcl-2, cyclin D1, p53 and rasp-21 genes was also studied. A significant (P
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2011.09.003