PRaTo: A web-tool to select optimal primer pairs for qPCR

► Outstanding primer pairs are a pre-requisite for good qPCR analysis. ► PRaTo web-tool was developed to check and rank primer pairs. ► PRaTo functionality was validated analyzing a qPCR primer repository. An essential pre-requisite to perform sound quantitative real-time polymerase chain reaction (...

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Veröffentlicht in:Biochemical and biophysical research communications 2011-12, Vol.415 (4), p.707-708
Hauptverfasser: Nonis, Alberto, Scortegagna, Marco, Nonis, Alessandro, Ruperti, Benedetto
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Sprache:eng
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Zusammenfassung:► Outstanding primer pairs are a pre-requisite for good qPCR analysis. ► PRaTo web-tool was developed to check and rank primer pairs. ► PRaTo functionality was validated analyzing a qPCR primer repository. An essential pre-requisite to perform sound quantitative real-time polymerase chain reaction (qPCR) assays is to design outstanding primer pairs. This means they must have a good efficiency and be not prone to produce multiple amplicons or primer dimer products. To circumvent these issues, several softwares are available to help primer design. Although satisfactory computer-aided primer design tools are available for standard PCR, less efforts were done to provide specific methods for selection of optimal primer pairs for qPCR. We have developed PRaTo a web-based tool that enables checking and ranking of primers pairs for their attitude to perform optimally and reliably when used in qPCR experiments. PRaTo is available at http://prato.daapv.unipd.it.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2011.10.148