Surface Modification of Gelatin Nanoparticles with Polyethylenimine as Gene Vector

Surface Modification of Gelatin Nanoparticles with Polyethylenimine as Gene Vector

A novel carrier on balancing the transfection efficiency and minimizing cytotoxicity was designed. Gelatin cross-linked with 1.8 kDa of PEI (GA-PEI 1.8 k) formed stable complex and resulted in high positive ζ potential (42.47 mV) and buffering effect. These nanoparticles with N/P ratio of 30 give hi...

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Veröffentlicht in:Journal of nanomaterials 2011-01, Vol.2011 (2011), p.1-5
Hauptverfasser: Kuo, Wei-Ti, Huang, Sam Hong-Yi, Chou, Min-Ju, Wu, Meng-Chao, Huang, Yi-You
Format: Artikel
Sprache:eng
Schlagworte:
Atoms & subatomic particles
Deoxyribonucleic acid
DNA
Efficiency
Gene expression
Gene therapy
Investigations
Nanomaterials
Nanoparticles
Ratios
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Zusammenfassung:A novel carrier on balancing the transfection efficiency and minimizing cytotoxicity was designed. Gelatin cross-linked with 1.8 kDa of PEI (GA-PEI 1.8 k) formed stable complex and resulted in high positive ζ potential (42.47 mV) and buffering effect. These nanoparticles with N/P ratio of 30 give high transfection efficiency 2.12×104 RLU/μg protein and cell viability (86.4%). These modified GA-PEI nanoparticles, with high transfection efficiency and low cell toxicity, can be a potential gene vector in gene therapy.
ISSN:1687-4110
1687-4129
DOI:10.1155/2011/646538