Radio labeling, quality control and biodistribution of 99mTc-cefotaxime as an infection imaging agent

Cefotaxime, a cephalosporin antibiotic, used to treat bacterial infections was investigated to label with 99m Tc. Labeling was performed using sodium dithionite as a reducing agent at 100 °C for 10 min and radiochemical analysis involved ITLC and HPLC methods. The stability of labeled antibiotic was...

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Veröffentlicht in:Journal of radioanalytical and nuclear chemistry 2011, Vol.287 (1), p.21-25
Hauptverfasser: Mirshojaei, S. F., Gandomkar, M., Najafi, R., Sadat Ebrahimi, S. E., Babaei, M. H., Shafiei, A., Talebi, M. H.
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Sprache:eng
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Zusammenfassung:Cefotaxime, a cephalosporin antibiotic, used to treat bacterial infections was investigated to label with 99m Tc. Labeling was performed using sodium dithionite as a reducing agent at 100 °C for 10 min and radiochemical analysis involved ITLC and HPLC methods. The stability of labeled antibiotic was checked in the presence of human serum at 37 °C up to 24 h. The maximum radiolabeling yield was 92 ± 2%. Bacterial binding assay was performed with S. aureus and the in vivo distribution was studied in mice. Images showed minimal accumulation in non-target tissues, with an average target/non-target ratio of 2.89 ± 0.58.
ISSN:0236-5731
1588-2780
DOI:10.1007/s10967-010-0826-8