Effect of glucose, lactate and pyruvate concentrations on in vitro growth of goat granulosa cell

Carbohydrates are among the most influential of the numerous components of culture medium that affect metabolism and developmental potential. Glucose, lactate and pyruvate are required for the growth of oocytes and other follicular cells in vitro. The aim of this study was to determine the effects of different concentrations of glucose, lactate and pyruvate on promoting DNA synthesis of granulosa cells in a serum-free medium. Effects of glucose (0.75, 1.5 or 3 mM), pyruvate (0.1 or 0.33 mM) and L-lactate (3, 6 or 12 mM) concentrations in the maturation medium on the relative granulosa cell growth during metaphase II (MII) were examined in a 3 x 2 x 3 factorial design. The greatest relative granulosa cell growth response (p