In vitro investigation of the repellent effects of the essential oil of Lippia javanica on adults of Hyalomma marginatum rufipes

A suitable in vitro tick climbing repellency bioassay was developed to evaluate the repellent effects of the essential oil (n-hexane extracts of distillate) of Lippia javanica (Burm. F.) Spreng (Verbemaceae) on adults of Hyalomma marginatum rufipes Koch, 1844. The GC-MS analysis of the distillate of the essential oil of Lippia javanica revealed that myrcene (13.4%), 1,8-cineole (8%), dyhydrotagetone (7.9%), ipsenone (9.6%), bicyclo (3.1.1) heptanes-2-one (20.8%) and 2-butanone (13.3%) were the major constituents. A significant (P < 0.05) dose dependent repellency response was observed for the essential oil of L. javanica on adults of H. marginatum rufipes when data were subjected to Kruskal Wallis analysis.