Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe

A fluorescent DNA probe containing an anthracene group attached via an anucleosidic linker can identify all four DNA bases at a single site as well as the epigenetic modification C/5-MeC via a hybridisation sensing assay.

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Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2011-06, Vol.47 (23), p.6629-6631
Hauptverfasser:	Duprey, Jean-Louis H A, Zhao, Zheng-yun, Bassani, Dario M, Manchester, Jack, Vyle, Joseph S, Tucker, James H R
Format:	Artikel
Sprache:	eng
Schlagworte:	Anthracenes - chemistry Circular Dichroism Cytosine - chemistry DNA - chemistry DNA Methylation DNA Probes - chemistry Fluorescent Dyes - chemistry Nucleic Acid Hybridization - methods Spectrometry, Fluorescence
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Beschreibung
Zusammenfassung:	A fluorescent DNA probe containing an anthracene group attached via an anucleosidic linker can identify all four DNA bases at a single site as well as the epigenetic modification C/5-MeC via a hybridisation sensing assay.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c1cc11205h