Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity.

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2011-01, Vol.47 (33), p.9465-9467
Hauptverfasser: Zhang, Jiangyan, Li, Zhengping, Wang, Hui, Wang, Yucong, Jia, Hongxia, Yan, Jingli
Format: Artikel
Sprache:eng
Schlagworte:
DNA Probes - chemistry
Humans
Lung - metabolism
MicroRNAs - analysis
Nucleic Acid Hybridization
Real-Time Polymerase Chain Reaction - methods
Ribonucleotides - chemistry
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Zusammenfassung:It was first demonstrated that the DNA probe modified with ribonucleotides can be efficiently ligated by using miRNA as the template. With PCR amplification of the ligated DNA probe, as low as 0.2 fM target miRNAs can be detected with high specificity.
ISSN:1359-7345
1364-548X
DOI:10.1039/c1cc13466c