A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm

A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl-4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytopla...

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Veröffentlicht in:	Organic & biomolecular chemistry 2011-05, Vol.9 (10), p.3615-3618
Hauptverfasser:	Liu, Xin, Sun, Yuming, Zhang, Yuanhong, Miao, Fang, Wang, Guancong, Zhao, Hongshi, Yu, Xiaoqiang, Liu, Hong, Wong, Wai-Yeung
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbazoles - chemical synthesis Carbazoles - chemistry Cell Nucleolus - metabolism Cytoplasm - metabolism Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry HeLa Cells Humans Microscopy, Fluorescence - methods Photons Pyridinium Compounds - chemical synthesis Pyridinium Compounds - chemistry RNA - chemistry RNA - metabolism Spectrometry, Fluorescence - methods
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Beschreibung
Zusammenfassung:	A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl-4-vinylpyridinium iodine)-N-ethylcarbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.
ISSN:	1477-0520 1477-0539
DOI:	10.1039/c1ob05123g