Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity

The effects of ionic liquid media on the activity of lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF]. Only [EtMeN][MeSO] was an ex...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Green chemistry : an international journal and green chemistry resource : GC 2004-01, Vol.6 (9), p.483-487
Hauptverfasser: Madeira Lau, Rute, Sorgedrager, Menno J., Carrea, Giacomo, van Rantwijk, Fred, Secundo, Francesco, Sheldon, Roger A.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The effects of ionic liquid media on the activity of lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF]. Only [EtMeN][MeSO] was an exception, as dissolved CaLB maintained its activity in this solvent. By means of FT-IR spectroscopy, denaturation of CaLB was observed upon dissolution in ionic liquids in which the activity was low, whereas the conformation of enzyme dissolved in [EtMeN][MeSO] closely resembled the native one.
ISSN:1463-9262
1463-9270
DOI:10.1039/b405693k