Dissolution of Candida antarctica lipase B in ionic liquids: effects on structure and activity
The effects of ionic liquid media on the activity of lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF]. Only [EtMeN][MeSO] was an ex...
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Veröffentlicht in: | Green chemistry : an international journal and green chemistry resource : GC 2004-01, Vol.6 (9), p.483-487 |
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Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The effects of ionic liquid media on the activity of lipase B in a simple transesterification reaction were studied. In ionic liquids containing alkylsulfate, nitrate and lactate anions, which dissolved CaLB, the reaction was at least ten times slower than in [BMIm][BF]. Only [EtMeN][MeSO] was an exception, as dissolved CaLB maintained its activity in this solvent. By means of FT-IR spectroscopy, denaturation of CaLB was observed upon dissolution in ionic liquids in which the activity was low, whereas the conformation of enzyme dissolved in [EtMeN][MeSO] closely resembled the native one. |
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ISSN: | 1463-9262 1463-9270 |
DOI: | 10.1039/b405693k |