p53 binding protein 1 foci as a biomarker of DNA double strand breaks induced by ionizing radiation

p53 binding protein 1 foci as a biomarker of DNA double strand breaks induced by ionizing radiation

Foci of p53 binding protein 1 (53 BP1) have been used as a biomarker of DNA double-strand breaks (DSBs) in cells induced by ionizing radiations. 53 BP1 was shown to relocalize into foci shortly after irradiation, with the number of foci closely paralleling the number of DNA DSBs. However, consensus...

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Veröffentlicht in:Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment Accelerators, spectrometers, detectors and associated equipment, 2011-12, Vol.660 (1), p.116-120
Hauptverfasser: Ng, C.K.M., Wong, M.Y.P., Lam, R.K.K., Ho, J.P.Y., Chiu, S.K., Yu, K.N.
Format: Artikel
Sprache:eng
Schlagworte:
53 BP1
Alpha particle
Bysta effect
DNA
Radiation effect
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Zusammenfassung:Foci of p53 binding protein 1 (53 BP1) have been used as a biomarker of DNA double-strand breaks (DSBs) in cells induced by ionizing radiations. 53 BP1 was shown to relocalize into foci shortly after irradiation, with the number of foci closely paralleling the number of DNA DSBs. However, consensus on criteria in terms of the numbers of 53 BP1 foci to define cells damaged by direct irradiation or by bystander signals has not been reached, which is partly due to the presence of 53 BP1 also in normal cells. The objective of the present work was to study the changes in the distribution of cells with different numbers of 53 BP1 foci in a cell population after low-dose ionizing irradiation (
ISSN:0168-9002
1872-9576
DOI:10.1016/j.nima.2011.09.020