A novel real-time polymerase chain reaction for identification of Salmonella enterica subspecies enterica

Abstract Salmonella enterica subspecies enterica (subspecies I) causes the majority of infections in humans and homeothermic animals. We present a real-time polymerase chain reaction assay targeting the hilA gene that demonstrates 97.9% specificity and 99.9% sensitivity for rapid and reliable identi...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2011-06, Vol.70 (2), p.278-280
Hauptverfasser:	Hopkins, Katie L, Lawson, Andy J, Connell, Steve, Peters, Tansy M, de Pinna, Elizabeth
Format:	Artikel
Sprache:	eng
Schlagworte:	5′ Nuclease real-time PCR Animals Bacterial Proteins - genetics Bacteriological Techniques - methods Bacteriology Biological and medical sciences Fundamental and applied biological sciences. Psychology hilA Humans Infectious Disease Infectious diseases Internal Medicine Medical sciences Microbiology Miscellaneous Molecular Sequence Data Polymerase Chain Reaction - methods Salmonella enterica Salmonella enterica - classification Salmonella enterica - genetics Salmonella enterica - isolation & purification Salmonella enterica subspecies enterica Salmonella Infections - diagnosis Salmonella Infections - microbiology Salmonella Infections, Animal - diagnosis Salmonella Infections, Animal - microbiology Sensitivity and Specificity Sequence Analysis, DNA Trans-Activators - genetics
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Zusammenfassung:	Abstract Salmonella enterica subspecies enterica (subspecies I) causes the majority of infections in humans and homeothermic animals. We present a real-time polymerase chain reaction assay targeting the hilA gene that demonstrates 97.9% specificity and 99.9% sensitivity for rapid and reliable identification of subspecies I, offering savings in time and labor over traditional methods.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2011.01.015