Thyroid-stimulating hormone (TSH): Measurement of intracellular, secreted, and circulating hormone in Xenopus laevis and Xenopus tropicalis

Highlights ► Sensitive and specific measurement of TSH in Xenopus laevis and Xenopus tropicalis . ► Ability to measure intracellular, secreted, and circulating TSH. ► First report for direct measurement of circulating TSH in amphibians. ► Purification of X. laevis pituitary TSH.

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Bibliographische Detailangaben
Veröffentlicht in:	General and comparative endocrinology 2011-05, Vol.171 (3), p.319-325
Hauptverfasser:	Korte, Joseph J, Sternberg, Robin M, Serrano, Jose A, Thoemke, Kara R, Moen, Scott M, Lillegard, Kathryn E, Hornung, Michael W, Tietge, Joseph E, Degitz, Sigmund J
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acid sequences amphibians Animals antibodies blood serum Blotting, Western detection limit Electrophoresis, Polyacrylamide Gel ELISA Endocrinology & Metabolism Enzyme-Linked Immunosorbent Assay Pituitary Pituitary Gland - metabolism polyacrylamide gel electrophoresis quantitative analysis secretion Serum tadpoles thyroid hormones Thyroid-stimulating hormone (TSH) thyrotropin Thyrotropin - blood Thyrotropin - metabolism Thyrotropin - secretion Western blot Western blotting Xenopus - blood Xenopus - metabolism Xenopus laevis Xenopus laevis - blood Xenopus laevis - metabolism Xenopus tropicalis
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Beschreibung
Zusammenfassung:	Highlights ► Sensitive and specific measurement of TSH in Xenopus laevis and Xenopus tropicalis . ► Ability to measure intracellular, secreted, and circulating TSH. ► First report for direct measurement of circulating TSH in amphibians. ► Purification of X. laevis pituitary TSH.
ISSN:	0016-6480 1095-6840
DOI:	10.1016/j.ygcen.2011.02.017