Application of quantitative real-time PCR for rapid identification of Bacteroides fragilis group and related organisms in human wound samples

Our goal was to establish a quantitative real-time PCR (QRT-PCR) method to detect Bacteroides fragilis group and related organisms from clinical specimens. Compared to conventional anaerobic culture, QRT-PCR can provide accurate and more rapid detection and identification of B. fragilis group and si...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Anaerobe 2011-04, Vol.17 (2), p.64-68
Hauptverfasser:	Tong, Jia, Liu, Chengxu, Summanen, Paula, Xu, Huaxi, Finegold, Sydney M.
Format:	Artikel
Sprache:	eng
Schlagworte:	abscess Abscess - diagnosis Abscess - microbiology bacteria Bacteriological Techniques - methods Bacteroides caccae Bacteroides distasonis Bacteroides eggerthii Bacteroides fragilis Bacteroides fragilis - classification Bacteroides fragilis - genetics Bacteroides fragilis - isolation & purification Bacteroides fragilis group Bacteroides Infections - diagnosis Bacteroides Infections - microbiology Bacteroides merdae Bacteroides ovatus Bacteroides splanchnicus Bacteroides stercoris Bacteroides thetaiotaomicron Bacteroides uniformis Bacteroides vulgatus DNA DNA Primers - genetics DNA, Bacterial - genetics DNA, Ribosomal - genetics Humans nucleotide sequences Oligonucleotide Probes - genetics pathogens PCR Polymerase Chain Reaction - methods QRT-PCR quantitative polymerase chain reaction rapid methods ribosomal RNA RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Time Factors Wound Infection - diagnosis Wound Infection - microbiology Wound samples
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Our goal was to establish a quantitative real-time PCR (QRT-PCR) method to detect Bacteroides fragilis group and related organisms from clinical specimens. Compared to conventional anaerobic culture, QRT-PCR can provide accurate and more rapid detection and identification of B. fragilis group and similar species. B. fragilis group and related organisms are the most frequently isolated anaerobic pathogens from clinical samples. However, culture and phenotypic identification is quite time-consuming. We designed specific primers and probes based on the 16S rRNA gene sequences of Bacteroides caccae, Bacteroides eggerthii, B. fragilis, Bacteroides ovatus, Bacteroides stercoris, Bacteroides thetaiotaomicron, Bacteroides uniformis, Bacteroides vulgatus, Odoribacter splanchnicus ( Bacteroides splanchnicus), Parabacteroides distasonis ( Bacteroides distasonis) and Parabacteroides merdae ( Bacteroides merdae), and detected these species by means of QRT-PCR in 400 human surgical wound infection samples or closed abscesses. The target bacteria were detected from 31 samples (8%) by culture, but from 132 samples (33%) by QRT-PCR ( p-value
ISSN:	1075-9964 1095-8274
DOI:	10.1016/j.anaerobe.2011.03.004