Cytokine expression in healthy mucosal colonic biopsies taken at colonoscopy compared to sigmoidoscopy indicate differences in bacterial signalling

Introduction The role of the commensal flora in the maintenance of a healthy mucosa is now recognized1 but there remains a lack of data in relation to the healthy human colon. Array analyses by us, comparing paired samples from 28 patients taken at colonoscopy and sigmoidoscopy, have highlighted innate immune responses to be of particular interest. The aim of this study was to assess, in a larger cohort from the same intervention study, differences in IL1β, IL8 and CCL4 (MIP1β) expression between the two bowel preparation methods, where there are differences in bacterial exposure. Methods Normal mucosa from three groups of patients with: (1) no apparent colonic disease (healthy, n=60), (2) previous polyps (n=73) or (3) ulcerative colitis in remission (n=37) was investigated as part of The FishGastro intervention study.2 Biopsy samples taken 25 cm from the anal verge at both colonoscopy and sigmoidoscopy were compared. Gene expression was analysed by RT-PCR and normalised to POL2 expression. Cytokine profiles of additional biopsies were measured using Luminex technology.3 Data were analysed by ANOVA. Results Gene expression of IL1, IL8 and CCL4 were higher in samples taken at sigmoidoscopy than in those taken at colonoscopy (table 1). Levels were comparable for control and polyps patients. UC patients had elevated expression of cytokines in colonoscopy samples but not in sigmoidoscopy biopsies. Protein expression patterns were similar. Table 1 PTU-012 Gene expression of three cytokines differentially expressed at colonoscopy and sigmoidoscopy Expression at colonoscopy Gene Expression ratio sigmoidoscopy colonoscopy ‘Healthy’ ‘Polyps’ UC IL1B 4.26; 1.40–10.64** 0.91; 0.57–1.31 0.87; 0.44–1.36 1.21; 0.33–2.06‡ IL8 4.65; 1.00–14.41** 0.70; 0.27–1.40 0.71; 0.35–1.67 1.20; 0.32–4.56‡ CCL4 1.93; 0.90–4.19** 0.05; 0.39–0.60 0.17; 0.49–0.83 0.20; 0.31–0.84 Results are presented as medians' range. **p