Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53
In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression....
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| Veröffentlicht in: | FEBS letters 2011-01, Vol.585 (1), p.29-35 |
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| creator | Rho, Seung Bae Lee, Jeong Heon Park, Mi Sun Byun, Hyun-Jung Kang, Sokbom Seo, Sang-Soo Kim, Joo-Young Park, Sang-Yoon |
| description | In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression. Overexpression of TCTP in lung carcinoma cells reversed p53 mediated apoptosis and inhibition of TCTP expression by small interfering RNA increased apoptosis of lung carcinoma cells. Moreover, it was observed that TCTP overexpression promotes degradation of p53. These results clearly indicate that the interaction between TCTP and p53 prevents apoptosis by destabilizing p53. Thus, TCTP acts as a negative regulator of apoptosis in lung cancer.
MINT-8057107, MINT-8057116: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti bait coimmunoprecipitation (MI:0006)
MINT-8057141: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid pooling approach (MI:0398)
MINT-8057126: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti tag coimmunoprecipitation (MI:0007) MINT-8057160: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid (MI:0018) |
| doi_str_mv | 10.1016/j.febslet.2010.11.014 |
| format | Article |
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MINT-8057107, MINT-8057116: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti bait coimmunoprecipitation (MI:0006)
MINT-8057141: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid pooling approach (MI:0398)
MINT-8057126: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti tag coimmunoprecipitation (MI:0007) MINT-8057160: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid (MI:0018)</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2010.11.014</identifier><identifier>PMID: 21081126</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl-2H-tetrazolium bromide ; 4,6′-diamidino-2-phenylindole ; Amino Acid Sequence ; Apoptosis ; Apoptosis Regulatory Proteins - genetics ; Apoptosis Regulatory Proteins - metabolism ; Binding Sites - genetics ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Blotting, Western ; Cell Line, Tumor ; Cell Proliferation ; DAPI ; DNA Fragmentation ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; histamine releasing factor ; HRF ; Humans ; Immunoprecipitation ; Lung carcinoma cell ; Molecular Sequence Data ; MTT ; O-nitrophenyl β-d-galactopyranoside ; ONPG ; p53 ; Protein Binding ; Protein Interaction Mapping ; Protein Stability ; RNA Interference ; siRNA ; small interfering RNA ; TCTP ; terminal deoxynucleotidyl transferase nick end label ; Transfection ; translationally controlled tumor protein ; Tumor Suppressor Protein p53 - genetics ; Tumor Suppressor Protein p53 - metabolism ; TUNEL ; Two-Hybrid System Techniques ; Yeast two-hybrid</subject><ispartof>FEBS letters, 2011-01, Vol.585 (1), p.29-35</ispartof><rights>2010 Federation of European Biochemical Societies</rights><rights>FEBS Letters 585 (2011) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5275-80c4cd465b027fce6752d120b1dcab8d3c6cb5da8769cfe54b7c0234c8a83c413</citedby><cites>FETCH-LOGICAL-c5275-80c4cd465b027fce6752d120b1dcab8d3c6cb5da8769cfe54b7c0234c8a83c413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2010.11.014$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2010.11.014$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,1418,1434,3551,27929,27930,45579,45580,46000,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21081126$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rho, Seung Bae</creatorcontrib><creatorcontrib>Lee, Jeong Heon</creatorcontrib><creatorcontrib>Park, Mi Sun</creatorcontrib><creatorcontrib>Byun, Hyun-Jung</creatorcontrib><creatorcontrib>Kang, Sokbom</creatorcontrib><creatorcontrib>Seo, Sang-Soo</creatorcontrib><creatorcontrib>Kim, Joo-Young</creatorcontrib><creatorcontrib>Park, Sang-Yoon</creatorcontrib><title>Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression. Overexpression of TCTP in lung carcinoma cells reversed p53 mediated apoptosis and inhibition of TCTP expression by small interfering RNA increased apoptosis of lung carcinoma cells. Moreover, it was observed that TCTP overexpression promotes degradation of p53. These results clearly indicate that the interaction between TCTP and p53 prevents apoptosis by destabilizing p53. Thus, TCTP acts as a negative regulator of apoptosis in lung cancer.
MINT-8057107, MINT-8057116: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti bait coimmunoprecipitation (MI:0006)
MINT-8057141: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid pooling approach (MI:0398)
MINT-8057126: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti tag coimmunoprecipitation (MI:0007) MINT-8057160: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid (MI:0018)</description><subject>3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl-2H-tetrazolium bromide</subject><subject>4,6′-diamidino-2-phenylindole</subject><subject>Amino Acid Sequence</subject><subject>Apoptosis</subject><subject>Apoptosis Regulatory Proteins - genetics</subject><subject>Apoptosis Regulatory Proteins - metabolism</subject><subject>Binding Sites - genetics</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>DAPI</subject><subject>DNA Fragmentation</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>histamine releasing factor</subject><subject>HRF</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Lung carcinoma cell</subject><subject>Molecular Sequence Data</subject><subject>MTT</subject><subject>O-nitrophenyl β-d-galactopyranoside</subject><subject>ONPG</subject><subject>p53</subject><subject>Protein Binding</subject><subject>Protein Interaction Mapping</subject><subject>Protein Stability</subject><subject>RNA Interference</subject><subject>siRNA</subject><subject>small interfering RNA</subject><subject>TCTP</subject><subject>terminal deoxynucleotidyl transferase nick end label</subject><subject>Transfection</subject><subject>translationally controlled tumor protein</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><subject>TUNEL</subject><subject>Two-Hybrid System Techniques</subject><subject>Yeast two-hybrid</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS1ERbelPwGUG6csHjtOnBMqq5ZWqgoS27OVTCbCq2wcbKdo_z0Ou3AtkiXbozdvRt9j7B3wNXAoP-7WPbVhoLgWfKnBmkPxiq1AVzKXRalfsxVPpVxVtTxnFyHsePprqN-wcwFcA4hyxR6vx2jzZnJTdNFiNnkXyY7ZdrP9lqEbo3dDyOIPykJsWjvYeMhc_6cQ573zWZinyVMI6Tkp-Zad9c0Q6Op0X7Kn25vt5i5_-PrlfnP9kKMSlco1xwK7olQtF1WPVFZKdCB4Cx02re4kltiqrtFVWWNPqmgr5EIWqBstsQB5yT4cfdO-P2cK0extQBqGZiQ3B1PzChSX6byk1ELUXICSSamOSvQuBE-9mbzdN_5ggJuFudmZE3OzMDcAJgFOfe9PE-Z2T92_rr-Qk-DuKPhlBzr8n6u5vfksvi8BLvlBiq4GrpLVp6MVJbjPlrwJaGlE6qwnjKZz9oVtfwMCSKoz</recordid><startdate>20110103</startdate><enddate>20110103</enddate><creator>Rho, Seung Bae</creator><creator>Lee, Jeong Heon</creator><creator>Park, Mi Sun</creator><creator>Byun, Hyun-Jung</creator><creator>Kang, Sokbom</creator><creator>Seo, Sang-Soo</creator><creator>Kim, Joo-Young</creator><creator>Park, Sang-Yoon</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TO</scope><scope>H94</scope></search><sort><creationdate>20110103</creationdate><title>Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53</title><author>Rho, Seung Bae ; Lee, Jeong Heon ; Park, Mi Sun ; Byun, Hyun-Jung ; Kang, Sokbom ; Seo, Sang-Soo ; Kim, Joo-Young ; Park, Sang-Yoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5275-80c4cd465b027fce6752d120b1dcab8d3c6cb5da8769cfe54b7c0234c8a83c413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl-2H-tetrazolium bromide</topic><topic>4,6′-diamidino-2-phenylindole</topic><topic>Amino Acid Sequence</topic><topic>Apoptosis</topic><topic>Apoptosis Regulatory Proteins - genetics</topic><topic>Apoptosis Regulatory Proteins - metabolism</topic><topic>Binding Sites - genetics</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>DAPI</topic><topic>DNA Fragmentation</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>histamine releasing factor</topic><topic>HRF</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Lung carcinoma cell</topic><topic>Molecular Sequence Data</topic><topic>MTT</topic><topic>O-nitrophenyl β-d-galactopyranoside</topic><topic>ONPG</topic><topic>p53</topic><topic>Protein Binding</topic><topic>Protein Interaction Mapping</topic><topic>Protein Stability</topic><topic>RNA Interference</topic><topic>siRNA</topic><topic>small interfering RNA</topic><topic>TCTP</topic><topic>terminal deoxynucleotidyl transferase nick end label</topic><topic>Transfection</topic><topic>translationally controlled tumor protein</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><topic>TUNEL</topic><topic>Two-Hybrid System Techniques</topic><topic>Yeast two-hybrid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rho, Seung Bae</creatorcontrib><creatorcontrib>Lee, Jeong Heon</creatorcontrib><creatorcontrib>Park, Mi Sun</creatorcontrib><creatorcontrib>Byun, Hyun-Jung</creatorcontrib><creatorcontrib>Kang, Sokbom</creatorcontrib><creatorcontrib>Seo, Sang-Soo</creatorcontrib><creatorcontrib>Kim, Joo-Young</creatorcontrib><creatorcontrib>Park, Sang-Yoon</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rho, Seung Bae</au><au>Lee, Jeong Heon</au><au>Park, Mi Sun</au><au>Byun, Hyun-Jung</au><au>Kang, Sokbom</au><au>Seo, Sang-Soo</au><au>Kim, Joo-Young</au><au>Park, Sang-Yoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2011-01-03</date><risdate>2011</risdate><volume>585</volume><issue>1</issue><spage>29</spage><epage>35</epage><pages>29-35</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression. Overexpression of TCTP in lung carcinoma cells reversed p53 mediated apoptosis and inhibition of TCTP expression by small interfering RNA increased apoptosis of lung carcinoma cells. Moreover, it was observed that TCTP overexpression promotes degradation of p53. These results clearly indicate that the interaction between TCTP and p53 prevents apoptosis by destabilizing p53. Thus, TCTP acts as a negative regulator of apoptosis in lung cancer.
MINT-8057107, MINT-8057116: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti bait coimmunoprecipitation (MI:0006)
MINT-8057141: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid pooling approach (MI:0398)
MINT-8057126: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti tag coimmunoprecipitation (MI:0007) MINT-8057160: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid (MI:0018)</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>21081126</pmid><doi>10.1016/j.febslet.2010.11.014</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 2011-01, Vol.585 (1), p.29-35 |
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| subjects | 3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl-2H-tetrazolium bromide 4,6′-diamidino-2-phenylindole Amino Acid Sequence Apoptosis Apoptosis Regulatory Proteins - genetics Apoptosis Regulatory Proteins - metabolism Binding Sites - genetics Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Blotting, Western Cell Line, Tumor Cell Proliferation DAPI DNA Fragmentation Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism histamine releasing factor HRF Humans Immunoprecipitation Lung carcinoma cell Molecular Sequence Data MTT O-nitrophenyl β-d-galactopyranoside ONPG p53 Protein Binding Protein Interaction Mapping Protein Stability RNA Interference siRNA small interfering RNA TCTP terminal deoxynucleotidyl transferase nick end label Transfection translationally controlled tumor protein Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism TUNEL Two-Hybrid System Techniques Yeast two-hybrid |
| title | Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53 |
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