Anti-apoptotic protein TCTP controls the stability of the tumor suppressor p53

In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression....

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Veröffentlicht in:	FEBS letters 2011-01, Vol.585 (1), p.29-35
Hauptverfasser:	Rho, Seung Bae, Lee, Jeong Heon, Park, Mi Sun, Byun, Hyun-Jung, Kang, Sokbom, Seo, Sang-Soo, Kim, Joo-Young, Park, Sang-Yoon
Format:	Artikel
Sprache:	eng
Schlagworte:	3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyl-2H-tetrazolium bromide 4,6′-diamidino-2-phenylindole Amino Acid Sequence Apoptosis Apoptosis Regulatory Proteins - genetics Apoptosis Regulatory Proteins - metabolism Binding Sites - genetics Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Blotting, Western Cell Line, Tumor Cell Proliferation DAPI DNA Fragmentation Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism histamine releasing factor HRF Humans Immunoprecipitation Lung carcinoma cell Molecular Sequence Data MTT O-nitrophenyl β-d-galactopyranoside ONPG p53 Protein Binding Protein Interaction Mapping Protein Stability RNA Interference siRNA small interfering RNA TCTP terminal deoxynucleotidyl transferase nick end label Transfection translationally controlled tumor protein Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism TUNEL Two-Hybrid System Techniques Yeast two-hybrid
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Zusammenfassung:	In this study, we identified p53 as a novel TCTP-interacting protein using TCTP as bait. Also, we determined the critical binding sites between TCTP and p53. To elucidate the functional consequence of the interaction, we developed the overexpression and inhibition system of TCTP and p53 expression. Overexpression of TCTP in lung carcinoma cells reversed p53 mediated apoptosis and inhibition of TCTP expression by small interfering RNA increased apoptosis of lung carcinoma cells. Moreover, it was observed that TCTP overexpression promotes degradation of p53. These results clearly indicate that the interaction between TCTP and p53 prevents apoptosis by destabilizing p53. Thus, TCTP acts as a negative regulator of apoptosis in lung cancer. MINT-8057107, MINT-8057116: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti bait coimmunoprecipitation (MI:0006) MINT-8057141: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid pooling approach (MI:0398) MINT-8057126: p53 (uniprotkb:P04637) physically interacts (MI:0915) with TCTP (uniprotkb:P13693) by anti tag coimmunoprecipitation (MI:0007) MINT-8057160: TCTP (uniprotkb:P13693) physically interacts (MI:0915) with p53 (uniprotkb:P04637) by two hybrid (MI:0018)
ISSN:	0014-5793 1873-3468
DOI:	10.1016/j.febslet.2010.11.014