A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins

Molecular engineering allows stoichiometric and co-localized expression of two optogenetic actuators, spaced by a fluorescent protein and an additional transmembrane helix in a single protein fusion. The method provides modular optogenetic tools for bidirectional membrane potential control or synerg...

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Veröffentlicht in:	Nature methods 2011-12, Vol.8 (12), p.1083-1088
Hauptverfasser:	Kleinlogel, Sonja, Terpitz, Ulrich, Legrum, Barbara, Gökbuget, Deniz, Boyden, Edward S, Bamann, Christian, Wood, Phillip G, Bamberg, Ernst
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2253 631/378/1697/1635 631/553/338 Artificial Gene Fusion Bacteriorhodopsins - analysis Bacteriorhodopsins - biosynthesis Bacteriorhodopsins - genetics Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biophysics Cells Gene expression Genes Genetic engineering HEK293 Cells Hippocampus - cytology Humans Life Sciences Light Membrane proteins Membranes Molecular Sequence Data Neurons - cytology Neurons - metabolism Physiological aspects Physiology Proteins Proteomics Rhodopsin - analysis Rhodopsin - biosynthesis Rhodopsin - genetics Volvox carteri
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