NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes

NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyo...

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Veröffentlicht in:	Nature methods 2011-10, Vol.8 (12), p.1037-1040
Hauptverfasser:	Elliott, David A, Braam, Stefan R, Koutsis, Katerina, Ng, Elizabeth S, Jenny, Robert, Lagerqvist, Ebba L, Biben, Christine, Hatzistavrou, Tanya, Hirst, Claire E, Yu, Qing C, Skelton, Rhys J P, Ward-van Oostwaard, Dorien, Lim, Sue Mei, Khammy, Ouda, Li, Xueling, Hawes, Susan M, Davis, Richard P, Goulburn, Adam L, Passier, Robert, Prall, Owen W J, Haynes, John M, Pouton, Colin W, Kaye, David M, Mummery, Christine L, Elefanty, Andrew G, Stanley, Edouard G
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Differentiation - genetics Antigens, Differentiation - metabolism Biomarkers - analysis Cell Differentiation Cell Separation - methods Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Gene Expression Profiling Green Fluorescent Proteins - metabolism Homeobox Protein Nkx-2.5 Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Myoblasts, Cardiac - cytology Myoblasts, Cardiac - metabolism Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism Polymerase Chain Reaction Receptors, Immunologic - genetics Receptors, Immunologic - metabolism Transcription Factors - genetics Transcription Factors - metabolism Vascular Cell Adhesion Molecule-1 - genetics Vascular Cell Adhesion Molecule-1 - metabolism
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Zusammenfassung:	NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
ISSN:	1548-7105
DOI:	10.1038/nmeth.1740