Cell growth and P(3HB) accumulation from CO2 of a carbon monoxide-tolerant hydrogen-oxidizing bacterium, Ideonella sp. O-1
Cell growth and accumulation of polyhydroxybutyric acid, P(3HB), from CO 2 in autotrophic condition of a newly isolated hydrogen-oxidizing bacterium, the strain O-1, was investigated. The bacterium, which was deposited in the Japan Collection of Microorganisms as JCM17105, autotrophically grows by a...
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Veröffentlicht in: | Applied microbiology and biotechnology 2011-12, Vol.92 (6), p.1161-1169 |
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Sprache: | eng |
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Zusammenfassung: | Cell growth and accumulation of polyhydroxybutyric acid, P(3HB), from CO
2
in autotrophic condition of a newly isolated hydrogen-oxidizing bacterium, the strain O-1, was investigated. The bacterium, which was deposited in the Japan Collection of Microorganisms as JCM17105, autotrophically grows by assimilating H
2
, O
2
, and CO
2
as substrate. 16S rRNA gene sequence of the bacterium was the closest to
Ideonella dechloratans
(99%). Specific growth rate of the strain O-1 was faster than a hydrogen-oxidizing bacterium,
Ralstonia eutropha
, which is well-known P(3HB)-producing microorganism. The strain O-1 is tolerant to high O
2
concentration and it can grow above 30% (
v
/
v
) O
2
, while the growth of
R. eutropha
and
Alcaligenes latus
was seriously inhibited. In culture medium containing 1 g/L (NH
4
)
2
SO
4
, cell concentration of the strain O-1 and P(3HB) increased to 6.75 and 5.26 g/L, respectively. The content of P(3HB) in the cells was 77.9% (
w
/
w
). The strain O-1 was very tolerant to carbon monoxide (CO) and it grew even at 70% (
v
/
v
) CO, while the growth of
R. eutropha
and
A. latus
were seriously inhibited at 5% (
v
/
v
) CO. From these results, it is expected that the strain O-1 will be useful in the manufacture of P(3HB) because the industrial exhaust gas containing CO
2
, H
2
, and CO can be directly used as the substrate in the fermentation process. |
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ISSN: | 0175-7598 1432-0614 |
DOI: | 10.1007/s00253-011-3420-2 |