Cytocompatibility of new bioceramic-based materials on human fibroblast cells (MRC-5)
Objective The aim of this study was to evaluate and compare the cytotoxicity of 2 bioceramic-based materials, BioAggregate and iRoot, on human fibroblast MRC-5 cells. Study design Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture...
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| Veröffentlicht in: | Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2011-12, Vol.112 (6), p.e137-e142 |
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| container_title | Oral surgery, oral medicine, oral pathology, oral radiology and endodontics |
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| creator | Mukhtar-Fayyad, Dalia, BDS, MSc, PhD |
| description | Objective The aim of this study was to evaluate and compare the cytotoxicity of 2 bioceramic-based materials, BioAggregate and iRoot, on human fibroblast MRC-5 cells. Study design Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture medium at 37°C for 5 days, and the extracts obtained were diluted with Dulbecco's Modified Eagle medium to achieve 5 concentrations (neat, 1/2, 1/10, 1/50, 1/100). Human fibroblast cells were incubated with each extract dilution for 24 hours, 72 hours, and 7 days, then cellular viability was assessed using MTT assay. Differences in mean cell viability values between materials were assessed by using the 1-way ANOVA and Tukey's test. Results A statistically significant difference was found between the experimental materials and the control group and between the 2 materials at the neat, 1/2, 1/10 concentrations for the 3 incubation periods ( P < .001). The cytotoxic effect of both materials was concentration dependant. Conclusions Both BioAggregate and iRoot SP displayed an acceptable biocompatibility. |
| doi_str_mv | 10.1016/j.tripleo.2011.05.042 |
| format | Article |
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Study design Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture medium at 37°C for 5 days, and the extracts obtained were diluted with Dulbecco's Modified Eagle medium to achieve 5 concentrations (neat, 1/2, 1/10, 1/50, 1/100). Human fibroblast cells were incubated with each extract dilution for 24 hours, 72 hours, and 7 days, then cellular viability was assessed using MTT assay. Differences in mean cell viability values between materials were assessed by using the 1-way ANOVA and Tukey's test. Results A statistically significant difference was found between the experimental materials and the control group and between the 2 materials at the neat, 1/2, 1/10 concentrations for the 3 incubation periods ( P < .001). The cytotoxic effect of both materials was concentration dependant. Conclusions Both BioAggregate and iRoot SP displayed an acceptable biocompatibility.</description><identifier>ISSN: 1079-2104</identifier><identifier>EISSN: 1528-395X</identifier><identifier>DOI: 10.1016/j.tripleo.2011.05.042</identifier><identifier>PMID: 21873087</identifier><language>eng</language><publisher>United States: Mosby, Inc</publisher><subject>Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Calcium Compounds - chemistry ; Calcium Compounds - pharmacology ; Calcium Hydroxide - chemistry ; Calcium Hydroxide - pharmacology ; Calcium Phosphates - chemistry ; Calcium Phosphates - pharmacology ; Cell Line ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Ceramics - chemistry ; Coloring Agents ; Culture Media ; Dentistry ; Durapatite - chemistry ; Durapatite - pharmacology ; Fibroblasts - drug effects ; Humans ; Humidity ; Hydroxyapatites - chemistry ; Hydroxyapatites - pharmacology ; Nanocomposites - chemistry ; Root Canal Filling Materials - chemistry ; Root Canal Filling Materials - pharmacology ; Silicates - chemistry ; Silicates - pharmacology ; Surgery ; Temperature ; Tetrazolium Salts ; Thiazoles ; Time Factors</subject><ispartof>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics, 2011-12, Vol.112 (6), p.e137-e142</ispartof><rights>Mosby, Inc.</rights><rights>2011 Mosby, Inc.</rights><rights>Copyright © 2011 Mosby, Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-e5dfd398306809e35ce783105dddd21f4674c20578902bf240546e192256bdc43</citedby><cites>FETCH-LOGICAL-c485t-e5dfd398306809e35ce783105dddd21f4674c20578902bf240546e192256bdc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1079210411003714$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21873087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mukhtar-Fayyad, Dalia, BDS, MSc, PhD</creatorcontrib><title>Cytocompatibility of new bioceramic-based materials on human fibroblast cells (MRC-5)</title><title>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</title><addtitle>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</addtitle><description>Objective The aim of this study was to evaluate and compare the cytotoxicity of 2 bioceramic-based materials, BioAggregate and iRoot, on human fibroblast MRC-5 cells. Study design Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture medium at 37°C for 5 days, and the extracts obtained were diluted with Dulbecco's Modified Eagle medium to achieve 5 concentrations (neat, 1/2, 1/10, 1/50, 1/100). Human fibroblast cells were incubated with each extract dilution for 24 hours, 72 hours, and 7 days, then cellular viability was assessed using MTT assay. Differences in mean cell viability values between materials were assessed by using the 1-way ANOVA and Tukey's test. Results A statistically significant difference was found between the experimental materials and the control group and between the 2 materials at the neat, 1/2, 1/10 concentrations for the 3 incubation periods ( P < .001). The cytotoxic effect of both materials was concentration dependant. Conclusions Both BioAggregate and iRoot SP displayed an acceptable biocompatibility.</description><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Calcium Compounds - chemistry</subject><subject>Calcium Compounds - pharmacology</subject><subject>Calcium Hydroxide - chemistry</subject><subject>Calcium Hydroxide - pharmacology</subject><subject>Calcium Phosphates - chemistry</subject><subject>Calcium Phosphates - pharmacology</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Ceramics - chemistry</subject><subject>Coloring Agents</subject><subject>Culture Media</subject><subject>Dentistry</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Fibroblasts - drug effects</subject><subject>Humans</subject><subject>Humidity</subject><subject>Hydroxyapatites - chemistry</subject><subject>Hydroxyapatites - pharmacology</subject><subject>Nanocomposites - chemistry</subject><subject>Root Canal Filling Materials - chemistry</subject><subject>Root Canal Filling Materials - pharmacology</subject><subject>Silicates - chemistry</subject><subject>Silicates - pharmacology</subject><subject>Surgery</subject><subject>Temperature</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Time Factors</subject><issn>1079-2104</issn><issn>1528-395X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuP1DAMgCMEYpeFnwDqDTi0OK-muYDQiJe0CAlYiVuUpq7I0DazSQqaf0-qGThwIRdHymc7_kzIYwoNBdq-2Dc5-sOEoWFAaQOyAcHukEsqWVdzLb_dLXdQumYUxAV5kNIeAFqu9X1ywWinOHTqktzsjjm4MB9s9r2ffD5WYawW_FX1PjiMdvau7m3CoZptxujtlKqwVN_X2S7V6PsY-smmXDmcysuzj593tXz-kNwbC4iPzvGK3Lx983X3vr7-9O7D7vV17UQnc41yGAeuOw5tBxq5dKg6TkEO5TA6ilYJx0CqTgPrRyZAihapZky2_eAEvyJPT3UPMdyumLKZfdp-YhcMazIaZKukkrKQ8kS6GFKKOJpD9LONR0PBbELN3pyFmk2oAWmK0JL35Nxh7Wcc_mb9MViAVycAy5w_PUaTnMfF4eAjumyG4P_b4uU_FdzkF-_s9AOPmPZhjUuRaKhJzID5sm11WyqlAFxRwX8DU_udiw</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Mukhtar-Fayyad, Dalia, BDS, MSc, PhD</creator><general>Mosby, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20111201</creationdate><title>Cytocompatibility of new bioceramic-based materials on human fibroblast cells (MRC-5)</title><author>Mukhtar-Fayyad, Dalia, BDS, MSc, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-e5dfd398306809e35ce783105dddd21f4674c20578902bf240546e192256bdc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Calcium Compounds - chemistry</topic><topic>Calcium Compounds - pharmacology</topic><topic>Calcium Hydroxide - chemistry</topic><topic>Calcium Hydroxide - pharmacology</topic><topic>Calcium Phosphates - chemistry</topic><topic>Calcium Phosphates - pharmacology</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Ceramics - chemistry</topic><topic>Coloring Agents</topic><topic>Culture Media</topic><topic>Dentistry</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Fibroblasts - drug effects</topic><topic>Humans</topic><topic>Humidity</topic><topic>Hydroxyapatites - chemistry</topic><topic>Hydroxyapatites - pharmacology</topic><topic>Nanocomposites - chemistry</topic><topic>Root Canal Filling Materials - chemistry</topic><topic>Root Canal Filling Materials - pharmacology</topic><topic>Silicates - chemistry</topic><topic>Silicates - pharmacology</topic><topic>Surgery</topic><topic>Temperature</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mukhtar-Fayyad, Dalia, BDS, MSc, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mukhtar-Fayyad, Dalia, BDS, MSc, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytocompatibility of new bioceramic-based materials on human fibroblast cells (MRC-5)</atitle><jtitle>Oral surgery, oral medicine, oral pathology, oral radiology and endodontics</jtitle><addtitle>Oral Surg Oral Med Oral Pathol Oral Radiol Endod</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>112</volume><issue>6</issue><spage>e137</spage><epage>e142</epage><pages>e137-e142</pages><issn>1079-2104</issn><eissn>1528-395X</eissn><abstract>Objective The aim of this study was to evaluate and compare the cytotoxicity of 2 bioceramic-based materials, BioAggregate and iRoot, on human fibroblast MRC-5 cells. Study design Twenty-four disks of BioAggregate and iRoot SP materials were fabricated (2 × 5 mm). Disks were immersed in cell culture medium at 37°C for 5 days, and the extracts obtained were diluted with Dulbecco's Modified Eagle medium to achieve 5 concentrations (neat, 1/2, 1/10, 1/50, 1/100). Human fibroblast cells were incubated with each extract dilution for 24 hours, 72 hours, and 7 days, then cellular viability was assessed using MTT assay. Differences in mean cell viability values between materials were assessed by using the 1-way ANOVA and Tukey's test. Results A statistically significant difference was found between the experimental materials and the control group and between the 2 materials at the neat, 1/2, 1/10 concentrations for the 3 incubation periods ( P < .001). The cytotoxic effect of both materials was concentration dependant. Conclusions Both BioAggregate and iRoot SP displayed an acceptable biocompatibility.</abstract><cop>United States</cop><pub>Mosby, Inc</pub><pmid>21873087</pmid><doi>10.1016/j.tripleo.2011.05.042</doi></addata></record> |
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| issn | 1079-2104 1528-395X |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Calcium Compounds - chemistry Calcium Compounds - pharmacology Calcium Hydroxide - chemistry Calcium Hydroxide - pharmacology Calcium Phosphates - chemistry Calcium Phosphates - pharmacology Cell Line Cell Proliferation - drug effects Cell Survival - drug effects Ceramics - chemistry Coloring Agents Culture Media Dentistry Durapatite - chemistry Durapatite - pharmacology Fibroblasts - drug effects Humans Humidity Hydroxyapatites - chemistry Hydroxyapatites - pharmacology Nanocomposites - chemistry Root Canal Filling Materials - chemistry Root Canal Filling Materials - pharmacology Silicates - chemistry Silicates - pharmacology Surgery Temperature Tetrazolium Salts Thiazoles Time Factors |
| title | Cytocompatibility of new bioceramic-based materials on human fibroblast cells (MRC-5) |
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