Calcineurin subunit B is an immunostimulatory protein and acts as a vaccine adjuvant inducing protective cellular and humoral responses against pneumococcal infection

Highlights • We used human PBMC-derived and mice marrow-derived dendritic cells as models. • We demonstrated that recombinant human calcineurin subunit B (CnB) induced phenotypic maturation of human PBMC-DCs and increased the secretion of cytokines. • In vivo experiments, we used murine models to ex...

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Veröffentlicht in:	Immunology letters 2011-10, Vol.140 (1), p.52-58
Hauptverfasser:	Li, Jing, Hu, MinLing, Guo, JunXia, Su, ZhenYi, Wei, Qun
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvant Adjuvants Adjuvants, Immunologic - metabolism Allergy and Immunology Animals Antigens, Bacterial - administration & dosage Antigens, Bacterial - immunology Antigens, Bacterial - metabolism Bacterial Proteins - administration & dosage Bacterial Proteins - immunology Bacterial Proteins - metabolism Calcineurin Calcineurin - administration & dosage Calcineurin - genetics Calcineurin - metabolism Calcineurin subunit B CD40 antigen CD80 antigen CD83 antigen CD86 antigen Cells, Cultured Dendritic cells Disease Models, Animal gamma -Interferon Helper cells Histocompatibility antigen HLA Humans Immune response Immune response (humoral) Immunity Immunity, Cellular - drug effects Immunity, Humoral - drug effects Immunization Immunoglobulin G Immunostimulation Infection Inflammation Interleukin 1 Interleukin 10 Interleukin 4 Interleukin 6 Lymphocytes T Mice Mice, Inbred BALB C Pneumococcal Infections - immunology Pneumococcal Infections - prevention & control pneumolysin Recombinant Proteins - administration & dosage Recombinant Proteins - immunology Recombinant Proteins - metabolism Splenocytes Streptococcal Vaccines Streptococcus pneumoniae Streptococcus pneumoniae - immunology Streptococcus pneumoniae - pathogenicity Streptolysins - administration & dosage Streptolysins - immunology Streptolysins - metabolism Th1-Th2 Balance - drug effects Transcription Tumor necrosis factor- alpha Vaccines
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Zusammenfassung:	Highlights • We used human PBMC-derived and mice marrow-derived dendritic cells as models. • We demonstrated that recombinant human calcineurin subunit B (CnB) induced phenotypic maturation of human PBMC-DCs and increased the secretion of cytokines. • In vivo experiments, we used murine models to examine the adjuvant effect of CnB on recombinant pneumolysin antigen (PN) specific immune responses. • It effectively promoted mixed Th1/Th2 type immune response.
ISSN:	0165-2478 1879-0542
DOI:	10.1016/j.imlet.2011.06.004