Quantitative real-time polymerase chain reaction improves conventional microbiological diagnosis in an outbreak of brucellosis due to ingestion of unpasteurized goat cheese

Abstract Rapid diagnosis of individuals involved in brucellosis outbreaks can sometimes be difficult with conventional microbiological techniques. We analyzed, for the first time, the diagnostic yield of a real-time polymerase chain reaction (PCR) assay in a family outbreak of brucellosis due to con...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2011-11, Vol.71 (3), p.294-296
Hauptverfasser:	Colmenero, Juan D, Clavijo, Encarnación, Morata, Pilar, Bravo, María J, Queipo-Ortuño, María I
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Animals Bacterial diseases Biological and medical sciences Brucella melitensis - genetics Brucella melitensis - isolation & purification Brucellosis Brucellosis - diagnosis Brucellosis - epidemiology Cheese - microbiology Child Diagnosis Disease Outbreaks Eating Epidemiology Family Health Female Fundamental and applied biological sciences. Psychology Genes, Bacterial Goats Human bacterial diseases Humans Infectious Disease Infectious diseases Internal Medicine Male Medical sciences Microbiology Middle Aged Miscellaneous Outbreak Real-time PCR Real-Time Polymerase Chain Reaction Young Adult
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Zusammenfassung:	Abstract Rapid diagnosis of individuals involved in brucellosis outbreaks can sometimes be difficult with conventional microbiological techniques. We analyzed, for the first time, the diagnostic yield of a real-time polymerase chain reaction (PCR) assay in a family outbreak of brucellosis due to consumption of unpasteurized goat cheese. PCR correctly identified all symptomatic cases.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2011.06.016