High fat diet induces central obesity, insulin resistance and microvascular dysfunction in hamsters

Microvascular dysfunction is an early finding in obesity possibly related to co-morbidities like diabetes and hypertension. Therefore we have investigated changes on microvascular function, body composition, glucose and insulin tolerance tests (GTT and ITT) on male hamsters fed either with high fat (HFD, n=20) or standard (Control, n=21) diet during 16weeks. Total body fat and protein content were determined by carcass analysis, aorta eNOS and iNOS expression by immunoblotting assay and mean blood pressure (MAP) and heart rate (HR) by an arterial catheter. Microvascular reactivity in response to acetylcholine and sodium nitroprusside, functional capillary density (FCD), capillary recruitment induced by a hyperinsulinemic status and macromolecular permeability after 30min ischemia was assessed on either cheek pouch or cremaster muscle preparations. Compared to Control, HFD animals have shown increased visceral fat (6.0±0.8 vs. 13.8±0.6g/100g BW), impaired endothelial dependent vasodilatation, decreased FCD (11.3±1.3 vs. 6.8±1.2/field) and capillary recruitment during hyperinsulinemia and increased macromolecular permeability after ischemia/reperfusion (86.4±5.2 vs.105.2±5.1leaks/cm2), iNOS expression and insulin resistance. MAP, HR, endothelial independent vasodilatation and eNOS expression were not different between groups. Our results have shown that HFD elicits an increase on visceral fat deposition, microvascular dysfunction and insulin resistance in hamsters. ► We have studied microcirculatory effects of high fat diet in hamsters ► High fat diet blunted arteriolar response to acetylcholine and induced iNOS expression in aorta. ► High fat diet induced insulin resistance. ► High fat diet decreased capillary recruitment during hyperinsulinemia.