Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii

Grain hardness of wheat is determined by the hardness ( Ha )-locus region, which contains three friabilin-related genes: puroindoline-a ( Pina ), puroindoline-b ( Pinb ) and GSP - 1 . In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha -locus...

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Veröffentlicht in:Plant cell reports 2011-12, Vol.30 (12), p.2293-2301
Hauptverfasser: Suzuki, Go, Wada, Hideo, Goto, Hiromi, Nakano, Akiko, Oba, Haruna, Deno, Takuya, Rahman, Sadequr, Mukai, Yasuhiko
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Sprache:eng
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Zusammenfassung:Grain hardness of wheat is determined by the hardness ( Ha )-locus region, which contains three friabilin-related genes: puroindoline-a ( Pina ), puroindoline-b ( Pinb ) and GSP - 1 . In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha -locus region of Aegilops tauschii containing Pina and GSP - 1 genes by Agrobacterium -mediated transformation. To examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity of the T-DNAs in four independent T 2 lines by using fluorescence in situ hybridization (FISH) and DNA gel blot analyses. The transgenes, Pina and GSP - 1 , were stably expressed in endosperms of the T 3 and T 4 seeds at RNA and protein levels, indicating that the promoters and other regulatory elements on the wheat Ha -locus region function in rice, and that multigene transformation using a large genomic fragment is a useful strategy. The functional contribution of the transgene-derived friabilins to the rice endosperm structure was considered as an increase of spaces between compound starch granules, resulting in a high proportion of white turbidity seeds.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-011-1134-7