Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii
Grain hardness of wheat is determined by the hardness ( Ha )-locus region, which contains three friabilin-related genes: puroindoline-a ( Pina ), puroindoline-b ( Pinb ) and GSP - 1 . In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the Ha -locus...
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Veröffentlicht in: | Plant cell reports 2011-12, Vol.30 (12), p.2293-2301 |
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Zusammenfassung: | Grain hardness of wheat is determined by the
hardness
(
Ha
)-locus region, which contains three friabilin-related genes: puroindoline-a (
Pina
), puroindoline-b (
Pinb
) and
GSP
-
1
. In our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the
Ha
-locus region of
Aegilops tauschii
containing
Pina
and
GSP
-
1
genes by
Agrobacterium
-mediated transformation. To examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity of the T-DNAs in four independent T
2
lines by using fluorescence in situ hybridization (FISH) and DNA gel blot analyses. The transgenes,
Pina
and
GSP
-
1
, were stably expressed in endosperms of the T
3
and T
4
seeds at RNA and protein levels, indicating that the promoters and other regulatory elements on the wheat
Ha
-locus region function in rice, and that multigene transformation using a large genomic fragment is a useful strategy. The functional contribution of the transgene-derived friabilins to the rice endosperm structure was considered as an increase of spaces between compound starch granules, resulting in a high proportion of white turbidity seeds. |
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ISSN: | 0721-7714 1432-203X |
DOI: | 10.1007/s00299-011-1134-7 |