Rapid hyperspectral fluorescence lifetime imaging
We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less t...
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| Veröffentlicht in: | Microscopy research and technique 2007-05, Vol.70 (5), p.481-484 |
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| container_end_page | 484 |
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| container_issue | 5 |
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| container_title | Microscopy research and technique |
| container_volume | 70 |
| creator | Beule, Pieter De Owen, Dylan M. Manning, Hugh B. Talbot, Clifford B. Requejo-Isidro, Jose Dunsby, Christopher Mcginty, James Benninger, Richard K.P. Elson, Daniel S. Munro, Ian John Lever, M. Anand, Praveen Neil, Mark A.A. French, Paul M.W. |
| description | We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jemt.20434 |
| format | Article |
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| ispartof | Microscopy research and technique, 2007-05, Vol.70 (5), p.481-484 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Arteries - chemistry Arteries - ultrastructure Convallaria - chemistry Convallaria - ultrastructure Diagnostic Imaging - methods FLIM fluorescence lifetime imaging Humans hyperspectral imaging Microscopy, Fluorescence - methods Time Factors tissue autofluorescence |
| title | Rapid hyperspectral fluorescence lifetime imaging |
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