Rapid hyperspectral fluorescence lifetime imaging

Rapid hyperspectral fluorescence lifetime imaging

We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less t...

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Veröffentlicht in:Microscopy research and technique 2007-05, Vol.70 (5), p.481-484
Hauptverfasser: Beule, Pieter De, Owen, Dylan M., Manning, Hugh B., Talbot, Clifford B., Requejo-Isidro, Jose, Dunsby, Christopher, Mcginty, James, Benninger, Richard K.P., Elson, Daniel S., Munro, Ian, John Lever, M., Anand, Praveen, Neil, Mark A.A., French, Paul M.W.
Format: Artikel
Sprache:eng
Schlagworte:
Arteries - chemistry
Arteries - ultrastructure
Convallaria - chemistry
Convallaria - ultrastructure
Diagnostic Imaging - methods
FLIM
fluorescence lifetime imaging
Humans
hyperspectral imaging
Microscopy, Fluorescence - methods
Time Factors
tissue autofluorescence
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Zusammenfassung:We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high‐speed FLIM technology in a line‐scanning microscope. We demonstrate the acquisition of whole‐field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.20434