Analysis of cyclosporin A in dried blood spots using liquid chromatography tandem mass spectrometry

Dried blood spot sampling is a promising and patient friendly alternative for venous sampling. A liquid chromatography tandem mass spectrometer assay was developed for analyzing cyclosporin A in dried blood spots. Linearity ranged from 25 to 1440μg/L. Within and between run accuracy and precision we...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-05, Vol.877 (14-15), p.1595-1598
Hauptverfasser:	Wilhelm, A.J., den Burger, J.C.G., Vos, R.M., Chahbouni, A., Sinjewel, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, Liquid - methods Cyclosporin Cyclosporine - blood Dried blood spot Fingerprick Fundamental and applied biological sciences. Psychology General pharmacology Humans Medical sciences Pharmacology. Drug treatments Specimen Handling Tandem Mass Spectrometry - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Dried blood spot sampling is a promising and patient friendly alternative for venous sampling. A liquid chromatography tandem mass spectrometer assay was developed for analyzing cyclosporin A in dried blood spots. Linearity ranged from 25 to 1440μg/L. Within and between run accuracy and precision were within limits. The developed assay has a negligible matrix-effect and a recovery of 97%. The dried blood spots were stable during a period of at least 17 days in the refrigerator. The developed assay is suitable for analyzing cyclosporin A in dried blood spots.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2009.03.024