Identification of critical infection parameters to control helper-dependent adenoviral vector production

Helper-dependent adenoviral vectors (HDVs) are the most promising adenoviral vectors for gene therapy treatments as well as vaccination strategies. However, the lack of a robust and efficient strategy to produce the HDV at high titers constitutes a major obstacle hindering the use of this promising...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of biotechnology 2009-06, Vol.142 (2), p.142-150
Hauptverfasser: Dormond, E., Perrier, M., Kamen, A.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Helper-dependent adenoviral vectors (HDVs) are the most promising adenoviral vectors for gene therapy treatments as well as vaccination strategies. However, the lack of a robust and efficient strategy to produce the HDV at high titers constitutes a major obstacle hindering the use of this promising technology at the clinical level. The HDV production requires a double infection of a recombinase-expressing HEK293 cell line with the HDV and a helper virus (HV). To limit the contamination of viral HDV lots by HV, encapsidation of HV is prevented by the recombinase action. A real-time quantitative PCR assay was developed to accurately characterize the contamination of the final product by HV. Infection strategies to enhance the HDV yield and reduce the contamination by HV were investigated. The multiplicity of infection (MOI) was identified as a critical parameter to simultaneously improve the HDV yield and reduce the contamination by HV. HDV-to-HV MOI ratio dictated the HDV yield whereas the HV accumulation was controlled by the MOI of HV. Delaying infection with the HV did not improve the HDV yield.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2009.03.021