Fluorescence detection with high time resolution: From optical microscopy to simultaneous force and fluorescence spectroscopy

Picosecond time‐resolution fluorescence signal detection over many hours is possible using the time‐correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Microscopy research and technique 2007-05, Vol.70 (5), p.433-441
Hauptverfasser:	Gaiduk, Alexander, Kühnemuth, Ralf, Felekyan, Suren, Antonik, Matthew, Becker, Wolfgang, Kudryavtsev, Volodymyr, Sandhagen, Carl, Seidel, Claus A.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	atomic-force microscopy confocal imaging DNA fluorescence correlation spectroscopy multiparameter fluorescence detection optical microscopy photon distribution analysis single molecules single photon counting
Online-Zugang:	Volltext
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