Fluorescence detection with high time resolution: From optical microscopy to simultaneous force and fluorescence spectroscopy

Picosecond time‐resolution fluorescence signal detection over many hours is possible using the time‐correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time...

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Veröffentlicht in:Microscopy research and technique 2007-05, Vol.70 (5), p.433-441
Hauptverfasser: Gaiduk, Alexander, Kühnemuth, Ralf, Felekyan, Suren, Antonik, Matthew, Becker, Wolfgang, Kudryavtsev, Volodymyr, Sandhagen, Carl, Seidel, Claus A.M.
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Sprache:eng
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Zusammenfassung:Picosecond time‐resolution fluorescence signal detection over many hours is possible using the time‐correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time scale from picoseconds to seconds. Optical imaging techniques combined with TCSPC allow one to study the spatial distribution of fluorescence properties in solution and on a surface. Mechanical manipulation of a single macromolecule by means of an atomic‐force microscope makes it possible to detect fluorescence signal changes as a function of mechanical conformations of a fluorescent dye attached to a single DNA molecule. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.20430