Modulatory effect of plasma folate and polymorphisms in one-carbon metabolism on catecholamine methyltransferase (COMT) H108L associated oxidative DNA damage and breast cancer risk

The present study was aimed to investigate the modulatory role of plasma folate and eight putatively functional polymorphisms of one-carbon metabolism on catecholamine methyltransferase (COMT)-mediated oxidative DNA damage and breast cancer risk. Plasma folate and 8-oxo-2'-deoxyguanosine (8-oxo...

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Veröffentlicht in:	Indian journal of biochemistry & biophysics 2011-08, Vol.48 (4), p.283-289
Hauptverfasser:	Naushad, Shaik Mohammad, Pavani, Addepalli, Rupasree, Yedluri, Sripurna, Deepti, Gottumukkala, Suryanarayana Raju, Digumarti, Raghunadha Rao, Kutala, Vijay Kumar
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Breast Neoplasms - enzymology Breast Neoplasms - genetics Case-Control Studies Catechol O-Methyltransferase - genetics DNA Damage DNA Primers Female Folic Acid - blood Humans Oxidation-Reduction Polymerase Chain Reaction Polymorphism, Genetic Polymorphism, Restriction Fragment Length
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Zusammenfassung:	The present study was aimed to investigate the modulatory role of plasma folate and eight putatively functional polymorphisms of one-carbon metabolism on catecholamine methyltransferase (COMT)-mediated oxidative DNA damage and breast cancer risk. Plasma folate and 8-oxo-2'-deoxyguanosine (8-oxodG) were estimated by commercially available kits, while polymorphisms were screened by PCR-RFLP and PCR-AFLP methods. COMT H108L polymorphism showed independent association with breast cancer (OR: 1.73, 95% CI: 1.31-2.30). No significant interaction was observed between folate status and COMT genotype. Multifactor dimensionality reduction (MDR) analysis gave evidence for the significant epistatic (gene-gene) interactions (p
ISSN:	0301-1208