Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS

Archaea use a tRNA Ile with agmatine conjugated at the C34 position to decode the AUA codon. The enzyme that catalyzes this reaction, TiaS, has been previously identified, and it is now shown that it uses a novel kinase domain to hydrolyze ATP into AMP and pyrophosphate before phosphorylating itself and the tRNA on its way to conjugating the agmatine. The archaeal AUA-codon specific tRNA Ile contains 2-agmatinylcytidine (agm 2 C or agmatidine) at the anticodon wobble position (position 34). The formation of this essential modification is catalyzed by tRNA Ile -agm 2 C synthetase (TiaS) using agmatine and ATP as substrates. TiaS has a previously unknown catalytic domain, which we have named the Thr18-Cyt34 kinase domain (TCKD). Biochemical analyses of Archaeoglobus fulgidus TiaS and its mutants revealed that the TCKD first hydrolyzes ATP into AMP and pyrophosphate, then phosphorylates the C2 position of C34 with the γ-phosphate. Next, the amino group of agmatine attacks this position to release the phosphate and form agm 2 C. Notably, the TCKD also autophosphorylates the Thr18 of TiaS, which may be involved in agm 2 C formation. Thus, the unique kinase domain of TiaS catalyzes dual phosphorylation of protein and RNA substrates.